MmuINT0097687 @ mm9
Intron Retention
Gene
ENSMUSG00000057751 | Megf6
Description
multiple EGF-like-domains 6 [Source:MGI Symbol;Acc:MGI:1919351]
Coordinates
chr4:153623536-153625053:+
Coord C1 exon
chr4:153623536-153623661
Coord A exon
chr4:153623662-153624930
Coord C2 exon
chr4:153624931-153625053
Length
1269 bp
Sequences
Splice sites
5' ss Seq
TCCGTAAGT
5' ss Score
10.27
3' ss Seq
TCCATCCCTCTGGTATCCAGGGA
3' ss Score
5.64
Exon sequences
Seq C1 exon
CCATCAGCTCCTGCACTCTGGGAAATGGTGGCTGCCAGCATCAGTGTGTACAGCTCACCGTGACACAGCACCGCTGCCAGTGCCGGCCCCAGTACCAGCTGCAGGAGGATGGCAGGCGCTGTGTCC
Seq A exon
GTAAGTATTGTTTGTGCAGCTGGGAGAACGAGGGGGTCCAGGATTCAGGGGAGAAGCTGATGTGAGGATGGATACCTGGTGGTAGGAGGGAGGTGAGGGCTGCTGTGGGCCTGAGCTGCAGGCATGGTGAGTGCTACCAGGTTTTGGGTCCTCCGAACAGGAAGTTCAGGATGCAGACAGCATTCTGGCTCTCTAGGTGTCTGTCTGGGGTGTGGGTGGAGGAGCCAGCCAGGCTCTGGGACTTTAGAATGCCTGCCTAGGAGAAAGACTTTGCTAAAGGAGTGTGTGGGTCAGGACCAGAGTCCCCTCCCCCACATCATGTGGAAGTGGGACAGAGCAGGTGCTGAGACTCCCCAGCACAGGAGGGAGGGAGTGTTAATATGAGGGGGCCGAGGCCAGCCATGTGGGTGGAATGGCATGGGGTCTCAGGACTGGGAGATCACAAAATTAGGGGGCACAGGGCTGAATAGGTTACAGAGCTGGGGTTGTTAGTAGTTCACGGGGTCACAGGAGTGCGAAGGTTACAGGATGGAGGGAATCACAGAGCTGGAGCCCTGGGGATGCAAAGGGGGAGAGTTTGACAGGGCTGAGGGAGTTACGAGGTTGAGAGGGGTACAGGCTTTGGGGGGCAGTCATAGGCTTGGCTGATCACATGGGATCCTTGGGTCAGAGGACTGGGGATCACATGGTTTGGGGCTTCTGTTTCAACACTGTTTCTCGTTTTGTTTTGTTTGTTTTGTTTTGTCTTGTTTTGTTTTGTTTTGTTTTTGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACCCACTCTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCGCCTGCCTCTGCCTCCCAAGTGCTGGGATCAACACTGTTTCTCAAAGCAAGAGATCTGGGGGGAGCAGGCCAGGCTCACAGTCAGAGCTGACCTGGGCTGGCCACCCCCTTGCTGGAAGGCCGCCTTCCTGCTAGGCTTTTCTTCTTAACATGGTGCACATTCTTTGGGAGCCATGACACTTGAAAGTCGTGGGTTTGGTCGGGTGCTGACTCTGTTCTGGCAGAGCCTTAGGAGTCCCGACCTTGGCTATGGAGACTTACTGGGGAATAGAGACTTATTGGGGGAACTCAGAGCTGCCTCCAGCTGCGGGGGCATGGCACAGGCTGGAGCAGAGAGGAATTCCTGTACTGAAGGGGATTCTGAGTCGTCAGAGTCCCTTTCCCCATCTCTTTTCTCTGCCTCCATCCCTCTGGTATCCAG
Seq C2 exon
GGAGAAGCCCCTGTGCAGACGGCAATGGTGGCTGTATGCATACATGCCAGGAACTCCGGGGCCTTGCCCACTGTGGCTGCCACCCAGGTTACCAGCTTGCTGCAGACCGCAAAGCCTGTGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000057751-Megf6:NM_001162977:6
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF146701=FXa_inhibition=WD(100=86.0)
A:
NA
C2:
PF146701=FXa_inhibition=WD(100=85.7),PF0764510=EGF_CA=PU(0.1=0.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CATCAGCTCCTGCACTCTGG
R:
CTTCACAGGCTTTGCGGTCTG
Band lengths:
248-1517
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: