MmuINT0103328 @ mm9
Intron Retention
Gene
ENSMUSG00000060180 | Myh13
Description
myosin, heavy polypeptide 13, skeletal muscle [Source:MGI Symbol;Acc:MGI:1339967]
Coordinates
chr11:67144486-67146095:+
Coord C1 exon
chr11:67144486-67144597
Coord A exon
chr11:67144598-67146002
Coord C2 exon
chr11:67146003-67146095
Length
1405 bp
Sequences
Splice sites
5' ss Seq
CAGGTAAGG
5' ss Score
11.08
3' ss Seq
CTCTCTTGACTTGTTACTAGGGA
3' ss Score
5.77
Exon sequences
Seq C1 exon
TGGAGAATCTGGGGCTGGAAAGACAGTGAACACGAAGCGTGTCATCCAATACTTTGCAACAATTGCAGTCACCGGGGACAAGAAGAAAGAGCAGCAGCCGGGCAAAATGCAG
Seq A exon
GTAAGGAGTACTATGGTACCAAAGGCTTGTTAGAATAAATATACCTGAAATATTAGGTGCATATCAAAGGTATGAGAGATGCACACTCAAAAGAGATAGCTCAATAGGACCAGATTTTTTTTGGTCCCTTTGTGGTTTTATATAGAAAAACTTTCATACCATTGTGTACACATTTTTCTATAACTGAAGTATCCTCCAGTTCTCTGAAAATGCCAACCAAGATCCTACAGCTCAATTTAATTCTGACACTACCTTCAGGGACATAACATCACATCCCACAAGTTAAAGGCTCAGACTCCCAAGCCCCAGTTCCTACAACCTGTACTTCTAGCCAACTTCTTAAAAGTCAAGAGTTCCTGCCATCTTCTCTGTGGCTAAAGTGGCTCACAGAATGGGAGCTATGTTCCTTACATTGATTAATTTATCATAAAGGATAAAACTCAGGAACACTCAGAGGGAAGAGATGCACAGATTACTATATTGGAAGAGAAGTCAGGAGTTGCAATGCCCTTTATAGGCCTGTCACCTTCCAGGCCCTCCCTTGGTTCATCAATCTAGAATCTCTCTAAACCCCTCTGGTTAAGGATCCTCACAGGGGTTCCATTAGCTAGCGATCAGTGACCAGCTCAGACTTCACTCTCTCTCCCCCTAAACCTCCAAGCCTCTAATCATGTGAAGCCCTTGTCCTGAGGCTTCAGCTCAGCTGCAACACATATAACACCATATCATATCATTACTATACCACACCACACCACATAACTTCAGAAACCCCAAAGGTTTTAGGCGTGCCGGGAAACTGAACAAAGACCAAGATACATGTCTAAGTCACAGTCACATATGAGGTGGTCCTGTCTGTGCAGACCTGTGTCTACCTAGCACCAACTCCATGAGCAACAGCTGAGTGGTCTTGAACAACTTCACAGCGCCCTTTAAGGGAATGATTAGGAGAACCATGGGTTTTCAAAAGGGGACACACTCCAGAATCACCGAGAGGACTTCTTAACAAGGCTCGTGAGACTCTAGACTTAGGATTCCTTGCTGACTAGGTCTCAGGTGCACCTTCTCCTGTTTTACTGATGTGCATGTTCACAAAACAAAACAAAACAAAACATGATTTATGATGAAGCATCACTAGAAAAGGAAGCTTGTGGAAACAGAGGGAACAATGTTCCAGCCTGGAGTCTGCCACAGCCAGAAGTCACTCCAAGGCTACCCTAACAAAAGACTCTCCAGGAGGGGAACCCTGAAACTCCTCTGGAGGGCTATCCACACATCAGAACTGACAACTAGTCAGGGGTGATATCCCTCTTAGGACTCTGCCCAGAGCACGCAGTTTCCATCCACCAAACACCCATCCCAGAAGATAGCCCAGGTTCTCCCCATTCCTCTCTTGACTTGTTACTAG
Seq C2 exon
GGAACGCTGGAGGATCAGATCATCCAAGCCAACCCCCTGCTGGAGGCCTTTGGAAACGCCAAGACCGTGAGGAACGACAACTCCTCCAGATTT
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000060180-Myh13:NM_001081250:5
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.289 A=NA C2=0.097
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(5.4=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(4.4=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Rat
(rn6)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGAGAATCTGGGGCTGGAAA
R:
TGGAGGAGTTGTCGTTCCTCA
Band lengths:
200-1605
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: