Special

MmuINT0103433 @ mm10

Intron Retention

Gene
Description
myosin, heavy polypeptide 2, skeletal muscle, adult [Source:MGI Symbol;Acc:MGI:1339710]
Coordinates
chr11:67191348-67191795:+
Coord C1 exon
chr11:67191348-67191466
Coord A exon
chr11:67191467-67191598
Coord C2 exon
chr11:67191599-67191795
Length
132 bp
Sequences
Splice sites
5' ss Seq
AAGGTGATT
5' ss Score
5.88
3' ss Seq
AGATGTGAACATCTCCATAGGCC
3' ss Score
5.84
Exon sequences
Seq C1 exon
GTGAATTTTCCAGGCAGCTTGATGAGAAGGAAGCGCTGGTATCTCAGTTATCAAGGGGGAAGCAAGCATTCACTCAACAGATTGAGGAGCTAAAGAGGCAGCTTGAAGAGGAAGTAAAG
Seq A exon
GTGATTATTAATCTAAGGCTGATGGCTGCAATTTGTTTCACGAGTTGACCCTAGAAAGAGCCAGTTCCACCATCGTTAAGGGGACTGAGTATTTAAATATCTAGTACAATGAAGATGTGAACATCTCCATAG
Seq C2 exon
GCCAAGAACGCGCTGGCCCACGCCCTGCAGTCCTCCCGCCATGACTGTGACCTGCTGCGGGAACAGTACGAGGAGGAGCAGGAGTCTAAGGCTGAACTGCAGAGGGCGCTGTCCAAGGCCAACAGCGAGGTGGCCCAGTGGAGGACCAAATATGAGACGGATGCCATCCAGCGCACGGAGGAGCTGGAGGAAGCCAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000033196:ENSMUST00000018641:28
Average complexity
IR
Mappability confidence:
NA
Protein Impact

ORF disruption upon sequence inclusion

No structure available
Features
Disorder rate (Iupred):
  C1=0.175 A=NA C2=0.424
Domain overlap (PFAM):

C1:
PF0003816=Filament=PD(4.9=35.0),PF0157614=Myosin_tail_1=FE(4.5=100),PF0003816=Filament=PU(10.5=75.0)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(7.6=100),PF0003816=Filament=FE(22.8=100)


Main Inclusion Isoform:
NA


Main Skipping Isoform:


Other Inclusion Isoforms:
NA


Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Zebrafish
(danRer10)
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTTGATGAGAAGGAAGCGCT
R:
CATATTTGGTCCTCCACTGGGC
Band lengths:
257-389
Functional annotations
There are 0 annotated functions for this event


GENOMIC CONTEXT[edit]

INCLUSION PATTERN[edit]


SPECIAL DATASETS

  • Pre-implantation embryo development
  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types