Special

MmuINT0103458 @ mm10

Intron Retention

Gene
ENSMUSG00000020908 | Myh3
Description
myosin, heavy polypeptide 3, skeletal muscle, embryonic [Source:MGI Symbol;Acc:MGI:1339709]
Coordinates
chr11:67088818-67089311:+
Coord C1 exon
chr11:67088818-67089124
Coord A exon
chr11:67089125-67089240
Coord C2 exon
chr11:67089241-67089311
Length
116 bp
Sequences
Splice sites
5' ss Seq
ATGGTAACT
5' ss Score
6.06
3' ss Seq
GGCTCCTTTGTGTTTTGCAGCGG
3' ss Score
9.49
Exon sequences
Seq C1 exon
CCGATGGGCATCTTCTCCATCCTGGAAGAGGAGTGCATGTTCCCCAAGGCGACAGACACCTCCTTCAAGAACAAGCTGTATGACCAGCACCTGGGAAAGTCCAACAACTTCCAGAAACCTAAGGTGGTCAAAGGCAAGGCTGAAGCCCACTTCTCCCTGGTGCACTATGCGGGCACCGTGGACTACAGTGTCTCAGGCTGGCTGGAGAAGAACAAGGACCCCCTGAATGAGACCGTGGTGGGACTGTACCAGAAGTCCTCCAACAGACTCCTGGCACATCTCTATGCCACCTTCGCTACAACAGATG
Seq A exon
GTAACTAACGGATTTCAGACGCAGGGGCTTTGCCTTCACATAATCTCCCTTTCCCACAGCATGCAAAAGTCTCGATACATCCACTCACAGTGCCCGGGCTCCTTTGTGTTTTGCAG
Seq C2 exon
CGGACGGTGGGAAGAAGAAAGTCGCTAAGAAGAAAGGCTCTTCCTTCCAAACCGTCTCTGCACTGTTCAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000020908:ENSMUST00000108689:16
Average complexity
IR
Mappability confidence:
NA
Protein Impact





	

	
ORF disruption upon sequence inclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.000 A=NA C2=0.125
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0006316=Myosin_head=FE(15.0=100)

							
A:
NA

							
C2:
PF0006316=Myosin_head=FE(3.4=100)

						

					

				








    
Main Inclusion Isoform:
NA





     
                   









    
Main Skipping Isoform:
ENSMUSP00000007301





     
      









    
Other Inclusion Isoforms:
NA
          









    
Other Skipping Isoforms:
ENSMUSP00000104329, ENSMUSP00000131883
  



Associated events






Other assemblies


mm9




Conservation


Human
(hg38)
chr17:10642311-10642416 
ALTERNATIVE
HsaINT0107726
(MYH3)
Human
(hg19)
chr17:10545628-10545733 
ALTERNATIVE
HsaINT0107726
(MYH3)
Rat
(rn6)
chr10:53633228-53633343 
LOW PSI
RnoINT0096471
(Myh3)
Cow
(bosTau6)
chr19:30240925-30241031 
LOW PSI
BtaINT0097437
(MYHC-EMBRYONIC)
Chicken
(galGal4)
chr18:594939-595091 
Chicken
(galGal3)
chr18:605067-605176 
LOW PSI
GgaINT0127417
([1])
Zebrafish
(danRer10)
chr24:41069485-41069597 
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
GCACCTGGGAAAGTCCAACAA

				
R: 
CTTTCTTCTTCCCACCGTCCG

				
Band lengths: 
242-358

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Ribosome-engaged transcriptomes of neuronal types
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types





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