MmuINT0103469 @ mm10
Intron Retention
Gene
ENSMUSG00000020908 | Myh3
Description
myosin, heavy polypeptide 3, skeletal muscle, embryonic [Source:MGI Symbol;Acc:MGI:1339709]
Coordinates
chr11:67093667-67094863:+
Coord C1 exon
chr11:67093667-67093757
Coord A exon
chr11:67093758-67094473
Coord C2 exon
chr11:67094474-67094863
Length
716 bp
Sequences
Splice sites
5' ss Seq
CAGGTAGGA
5' ss Score
9.79
3' ss Seq
GGCATCCCCGCTCTCTTTAGGCT
3' ss Score
8.13
Exon sequences
Seq C1 exon
GAAGGACTTTGAATATAGTCAGCTGCAAAGCAAAGTGGAAGATGAGCAGACCTTGAGCCTCCAGCTTCAGAAGAAAATCAAGGAGCTACAG
Seq A exon
GTAGGAGGAGGTGGTCAGCTCTCGCTTGCCCGCGTGGCTCACTCAGAAACCTGAGCGGTGTCTGCTGAAGTCAGAGTAGGTAGGGTAGGACTGTGTGGCTGCCTCATGGGACCTCACTCTGTCAGGCTGACTTTGCAGTGGTGATGGTAATGACCGTGTCCCCCAAACTTGCAACAGAATGCATGGAGGTGGATGGAGAAATTTCCAGGGTAGCTAGTGACCATAATGGTGCAGGAAAAGATTCATTTGGAAGAAAATTAGTAGCATTTATGTTTCTCTGGGGGAACATCCATGAGCAGATTCATCTGGTGGGAACATAACCTCTTCTCCATCATCATCGTCGTCGTCATCACCTAATATTGTATATTAATAAGTTTCCATGTGCTAGTTCATCACGTGTTTATAGTGCTCCTCCACCCAGCCCCTTCTTAGCTACGTTCCCCACCTTCCTCGCCTCTACCAATCACCATTTCATATTCAGATTGACTTAAACAAATTCTTCACATGAAAAACACGCACCACTTGTTTTTCCAGTGTCTGGCGCCCTCCGTTTTGCTGCAAATGGCATGACTTCATTCTTTTTATAGCTGAGTCATATGCTACCATTTAAGTAAAAGATGTCTTCTTCATGCATTCGTGGATCGATGACACCTATTCAGATGCCATGTTAGCTATTGTGACCCTGAGAGTCTCTTGGGCATCCCCGCTCTCTTTAG
Seq C2 exon
GCTCGCATCGAGGAGCTGGAGGAAGAGATAGAGGCAGAGAGGGCCACCCGGGCCAAGACAGAGAAGCAGCGCAGCGACTATGCACGCGAGCTGGAGGAGCTGAGTGAGCGGCTGGAGGAGGCGGGCGGTGTCACCTCCACCCAGATCGAACTGAACAAGAAGCGTGAGGCTGAGTTCTTGAAGCTGCGCAGGGACCTGGAGGAGGCCACCCTGCAGCACGAGGCCACGGTGGCCACGCTGCGGAAGAAGCACGCGGACAGCGCGGCCGAGCTGGCGGAGCAGATCGACAACCTGCAGCGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGTTCAAGCTGGAGATTGACGACCTCTCCAGCAGCGTAGAGAGCGTGTCCAAATCCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000020908:ENSMUST00000108689:26
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.417
Domain overlap (PFAM):
C1:
PF0003816=Filament=FE(11.0=100),PF0157614=Myosin_tail_1=FE(9.2=100)
A:
NA
C2:
PF0003816=Filament=FE(47.4=100),PF0157614=Myosin_tail_1=FE(39.4=100),PF104734=CENP-F_leu_zip=PU(42.0=44.6)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGATGAGCAGACCTTGAGCCT
R:
CTGCAGGTTGTCGATCTGCTC
Band lengths:
349-1065
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types