MmuINT0103476 @ mm9
Intron Retention
Gene
ENSMUSG00000020908 | Myh3
Description
myosin, heavy polypeptide 3, skeletal muscle, embryonic [Source:MGI Symbol;Acc:MGI:1339709]
Coordinates
chr11:66911381-66912204:+
Coord C1 exon
chr11:66911381-66911546
Coord A exon
chr11:66911547-66912079
Coord C2 exon
chr11:66912080-66912204
Length
533 bp
Sequences
Splice sites
5' ss Seq
AACGTAAGC
5' ss Score
7.35
3' ss Seq
GCGAGCTCATTTCATTGCAGAAG
3' ss Score
4.67
Exon sequences
Seq C1 exon
GTGCTAGCTGAGTGGAAGACGAAGTGTGAGGAGAGCCAAGCCGAGCTGGAGGCAGCTCTCAAGGAATCCCGGTCCTTGAGCACTGAGCTCTTCAAACTGAAGAATGCCTATGAAGAAGCCTTGGATCAACTTGAAACCGTGAAACGGGAGAATAAGAACTTAGAAC
Seq A exon
GTAAGCCTTTCCGAACTGTCCGAGGTTGTGGGAAACCCTTCTTGTCACCCATCGTGGCCAGAGTGTGTGAGGGTGGATGAGACTCTGTTATTCAAGCTCCTCTACATGGGTCTGCTGATGCACCAATAGAAACTGTTCACTGTTGACAAGTTGGTTTTTCTTAAAGATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTATCTAATTTATATGGGTACGTTCTCTTCAGACACACCAGAAGAGGGCATGAGATCCCATTGCAGATGGTTGTGAGCCACCATGTGGTTGCTGGGAATTGAACTCAGGACCTCTGGAAGAGCAGTCAGTGCTTTTAACCGCTGAGCTATCTCTCCAACCCAGCAAGTTGTTGTTTTGAGTCTCTATACTATGTCTTAGCTCTGAGAAGCAGAGGTCTTTAGAGTTACAGAGAGCTAACGACAGGCATCTAGTGGGCGCTTCACATTCCCAGGAGCCTGTGCAGAGGCAGGCGTTCAGATGCAATGCGAGCTCATTTCATTGCAG
Seq C2 exon
AAGAGATAGCAGATCTCACTGAACAGATTGCCGAGAACGGTAAAAGCATCCATGAGCTAGAGAAATCCAGAAAACAGATGGAGCTGGAGAAGGCTGACATCCAGATGGCGCTGGAGGAAGCAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000020908-Myh3:NM_001099635:32
Average complexity
IR-S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.125 A=NA C2=0.571
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(6.4=100),PF045827=Reo_sigmaC=FE(36.9=100),PF152721=BBP1_C=FE(42.3=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(4.8=100),PF045827=Reo_sigmaC=PD(22.1=78.6),PF152721=BBP1_C=FE(31.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGCTAGCTGAGTGGAAGACG
R:
CTTCCTCCAGCGCCATCTG
Band lengths:
286-819
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: