MmuINT0103478 @ mm10
Intron Retention
Gene
ENSMUSG00000020908 | Myh3
Description
myosin, heavy polypeptide 3, skeletal muscle, embryonic [Source:MGI Symbol;Acc:MGI:1339709]
Coordinates
chr11:67099178-67099803:+
Coord C1 exon
chr11:67099178-67099486
Coord A exon
chr11:67099487-67099599
Coord C2 exon
chr11:67099600-67099803
Length
113 bp
Sequences
Splice sites
5' ss Seq
AAGGTGGAG
5' ss Score
1.51
3' ss Seq
CGCTCTTGGCTTCTGTCTAGGAC
3' ss Score
7.93
Exon sequences
Seq C1 exon
GCAGCTCTTGAGCACGAAGAAGCCAAGATTCTCCGGATCCAGCTTGAACTGACCCAGGTGAAATCAGAGATTGATAGGAAGATTGCAGAGAAGGATGAAGAGATCGAGCAGCTGAAGAGGAACTACCAGAGGACAGTGGAGACCATGCAAGGCGCCCTGGACGCTGAGGTGCGCAGCAGGAATGAGGCCATCCGCCTGAAGAAGAAGATGGAGGGCGACCTCAATGAGATCGAGATCCAGCTGAGCCACGCCAACCGCCAGGCCGCAGAGACCATCAAGCACCTCCGGAGCGTCCAGGGACAGCTGAAG
Seq A exon
GTGGAGGGAGCGTCCTGGGGTGGGGGGACTTTGCCAGTGTCCAGATATGAAAAGACTAGGCCAAGTTTGTGGTCCTTGGCTCTCCTCTTCACACGCTCTTGGCTTCTGTCTAG
Seq C2 exon
GACACCCAGCTGCACCTGGATGACGCTCTCCGGGGCCAGGAGGACCTGAAGGAGCAGCTGGCCATCGTGGAACGCAGAGCCAACCTCCTGCAGGCTGAGGTGGAGGAGCTGCGGGCCACGCTGGAGCAGACGGAGAGGGCCCGGAAGCTGGCAGAGCAGGAGCTGTTGGATTCGAATGAGAGGGTCCAGCTGCTGCACACCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000020908:ENSMUST00000108689:34
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.272 A=NA C2=0.426
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(11.9=100),PF152721=BBP1_C=PD(3.8=4.9)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(7.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GATCGAGATCCAGCTGAGCCA
R:
TTCGAATCCAACAGCTCCTGC
Band lengths:
258-371
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types