MmuINT0119730 @ mm10
Intron Retention
Gene
ENSMUSG00000026447 | Pik3c2b
Description
phosphoinositide-3-kinase, class 2, beta polypeptide [Source:MGI Symbol;Acc:MGI:2685045]
Coordinates
chr1:133074511-133074858:+
Coord C1 exon
chr1:133074511-133074622
Coord A exon
chr1:133074623-133074778
Coord C2 exon
chr1:133074779-133074858
Length
156 bp
Sequences
Splice sites
5' ss Seq
ACGGTACGT
5' ss Score
11.34
3' ss Seq
CTGCTCGCTCCCTCCCTCAGGTG
3' ss Score
11.31
Exon sequences
Seq C1 exon
TAAGCACGCCCTGGGAAGCCATGAGTATATCCAATATTGCCGCAAGTTTGATATCAGCATCCGGCTACAGCTGATGGAGCAGAAGGCTATCCGGAGCGACCTGGCCCGGACG
Seq A exon
GTACGTGTCGGAAATGGCTGACAGTTGTACATAGCTGGGCAGGTAGAATGGGCAGAGGGTGATATGGAACATGTTAAGGGAGTGGGGTAGGTATCCTGGGCTGAGGAAAGGGCGTCATCCCTGCCTGACCAGGGCACTGCTCGCTCCCTCCCTCAG
Seq C2 exon
GTGAATGACGACCAGAGCCCTTCCACCTTGAACTACCTCATCCATCTCCAAGAGAGGCCAGTCAAGCAGACCATCAGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000026447:ENSMUST00000077730:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.053 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0079413=PI3K_rbd=PD(28.2=76.3)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TAAGCACGCCCTGGGAAGC
R:
CTGATGGTCTGCTTGACTGGC
Band lengths:
189-345
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types