MmuINT0131351 @ mm10
Intron Retention
Gene
ENSMUSG00000026821 | Ralgds
Description
ral guanine nucleotide dissociation stimulator [Source:MGI Symbol;Acc:MGI:107485]
Coordinates
chr2:28542290-28542950:+
Coord C1 exon
chr2:28542290-28542483
Coord A exon
chr2:28542484-28542854
Coord C2 exon
chr2:28542855-28542950
Length
371 bp
Sequences
Splice sites
5' ss Seq
AAGGTGAGC
5' ss Score
9.6
3' ss Seq
ACACTAGACATCATAAGCAGGTA
3' ss Score
-0.5
Exon sequences
Seq C1 exon
TGTGAGAACGAGTCGGCTCTGAACCTCTATGAGACCTGCAAGGTGCGCACGGTGAAGGCTGGTACTCTGGAGAAGCTGGTGGAACACCTGGTGCCTGCCTTCCAGGGCAGTGACCTTTCCTACGTCACTGTCTTCCTGTGCACCTACAGAGCCTTCACTACCACCCAGCAGGTGCTAGACCTGCTGTTCAAAAG
Seq A exon
GTGAGCACTGCCGCCAGACCCAGGACACCCTTCCTGCCGGCCGCACCTTGGGGGAGCCCAGCTTGGGGATAAGCCTCCTTTCAGGCTCCTGTCAGGGTGGGCTGCCAGGGCCTTCCTCTCCTCAGCCTCAGGTTAAGTGGGTTCCCTACATAGAAAGGAGGGGACCTCAACCTAGAACACAGAGCAAGGACGGCCCTGTGGTAATCGACTGAGAGACCTCAGGTCTGCTGAAGTGCCTCGGTTTCTAAAGCAATCTGCTGTGCGTAGCCATGTTGCCAAGCACCTAACTCTGTGACAGGAGCTGAGTCCCTGCCTCCCTGGGCCCCATTCCCATCAGGGAGTTGACCAGTAACACTAGACATCATAAGCAG
Seq C2 exon
GTACGGTAGATGTGACGCCCTCACGGCCTCCTCTAGATATGGCTGCATCCTCCCCTACTCCAGTGAGGACGGCGGACCGCAGGACCAACTCAAAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000026821:ENSMUST00000028170:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.015
Domain overlap (PFAM):
C1:
PF0061815=RasGEF_N=PU(42.5=73.8)
A:
NA
C2:
PF0061815=RasGEF_N=FE(28.3=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGAGAACGAGTCGGCTCTGAA
R:
TTTTGAGTTGGTCCTGCGGTC
Band lengths:
287-658
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types