MmuINT0131996 @ mm10
Intron Retention
Gene
ENSMUSG00000004952 | Rasa4
Description
RAS p21 protein activator 4 [Source:MGI Symbol;Acc:MGI:1858600]
Coordinates
chr5:136102528-136103424:+
Coord C1 exon
chr5:136102528-136102665
Coord A exon
chr5:136102666-136103279
Coord C2 exon
chr5:136103280-136103424
Length
614 bp
Sequences
Splice sites
5' ss Seq
AAGGTGTGG
5' ss Score
5.28
3' ss Seq
GTTCTTGCTGTCATCCCTAGGCG
3' ss Score
10.15
Exon sequences
Seq C1 exon
AACGTACCATTCATCGCGGTCACCAGCTTCCTGTGCCTGCGCTTCTTCTCTCCCGCCATCCTGTCGCCCAAGCTCTTCCACCTGCGAGAGCGGCATGCAGATGCCCGCACCAGCCGCACGCTGCTTCTGCTGGCCAAG
Seq A exon
GTGTGGGCGGGCCTGCAGAGTGGAACGGGAAGGCCAGTTAGGTTGCAAGGGACCCTGGCAGGCTCTGTAGTGTCGCAACAATTGGCACTAGGAAGGTCACTGATCCGATATGGCACTGCACTTCACAGTTTAAAATAATGCTCGTGATCACTTCTTGAGAGCCCCATCCACACATAAGGAGACACAGAAAGCCCAAAGCCCAAATCTTCCCAGTTCTAATCTTATTCCCAGACATGCAGAAGTCTGATAGTAGGATGAAACTTTCAGGGCCAGCAAGAGGGCTTAGTAGGTAAGGGTGCTTGCTGCCAACCCTGGTGACCCGAGTTCCATCCTTGGAGCCCACATGATGGAAGGAGGGAACCAGCTCCCACAATCGGCATCCAAGTCCCACATGTGCCCAGGCAAGCCCCAACACACACGCAATGATAATATAATTAAACACATTTGCAGAGAAAAGCCGAAATGTTGATCCCAAAGTACCAGGTGTCCAAAGACTCAAAATGCCATGATCCCTGGTTCCCCTGTGGGACTTCGTAAGGCCTCAGCTTGAAGTAGGCAGCCATGGATGAGCTGTTGTCTCCCCAAAGCTCTATCGTTCTTGCTGTCATCCCTAG
Seq C2 exon
GCGGTCCAGAACATAGGCAATATGGACACACCGGTCTCTAGGGCCAAAGAGTCGTGGATGGAACCACTCCAGCCTACCGTGCGCCAGGGCGTGGCGCAGCTGAAGGACTTCATTATGAAGCTTGTGGACATAGAAGAAAAGGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000004952:ENSMUST00000042135:14
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.163
Domain overlap (PFAM):
C1:
PF0061614=RasGAP=FE(23.7=100)
A:
NA
C2:
PF0061614=RasGAP=PD(3.2=12.2)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AACGTACCATTCATCGCGGTC
R:
TCCTTTTCTTCTATGTCCACAAGCT
Band lengths:
281-895
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types