DreINT0124777 @ danRer10
Intron Retention
Gene
ENSDARG00000029372 | rasa4
Description
RAS p21 protein activator 4 [Source:ZFIN;Acc:ZDB-GENE-060526-349]
Coordinates
chr5:58698078-58698911:+
Coord C1 exon
chr5:58698078-58698215
Coord A exon
chr5:58698216-58698766
Coord C2 exon
chr5:58698767-58698911
Length
551 bp
Sequences
Splice sites
5' ss Seq
AAAGTATGA
5' ss Score
3.87
3' ss Seq
CCTCATGTATGTTTCCTCAGGCT
3' ss Score
7.94
Exon sequences
Seq C1 exon
AAGGTGAAGTTCATTGCTGTAACCAGCTTCCTCTGTCTGCGGTTCATCTCACCTGCCATCATGTCTCCAAAACTATTTCACCTACGAGAGAAACATGCCGATGCCCGTACCAGCCGTACACTCCTGCTACTTGCCAAA
Seq A exon
GTATGACCAATTGTTCCCGAAAACCATTGTTGTTGTGTATCCTGGTAACAGATAAGTTTCATACTACATTATATAGTTGTATTTGAGGATCAAAGTATGTCATTTGCATGTCCTGAGCTCTTATTATTTAGCTATGCCTAGATACATTCAGATTTTCATTCTATGGCACATTCAAAGTTTCATTACACGCTATTCACAAAACAAAAGAGGATAACAGATGATAATATGTTGTAATAACAACCACTATTTATGTAGGTCACACTTTCATCATAGATTATGACCTCCATCCATTATAGCAGTGTTTCCCTACCCTGTTTCTGAGGGTACACCCACAGTACACATTTTCAACCTCTCCCCAATCAAACACACCAGAATCAACTCATCAGAACGTAAGAAGAGACTCTAAAACCTGAAGTTAATGGGTTAGATAAGGGAGACATCCAAAATATGTACTGTTGGTGTGCCTCAGGAACAGGGTTGGAAAACACTGCATTACAGACTTCATTAAGGTTACCATATTCCCTTTGACCACCTCATGTATGTTTCCTCAG
Seq C2 exon
GCTGTGCAGACCATTGGGAACATGGACACTCTGGCCTGCTGCTCTAAAGAGCCGTGGATGGTGCGTTTGCAGCCTGCCATTCAGCAAGGCATCACCCAGCTCAAAGACTTCATCACCAGACTGGTCAACTGCCATGAGTCAGAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000029372:ENSDART00000097374:14
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0061614=RasGAP=FE(23.7=100)
A:
NA
C2:
PF0061614=RasGAP=PD(3.2=12.2)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGTGAAGTTCATTGCTGTAACCA
R:
TGACTCATGGCAGTTGACCAG
Band lengths:
278-829
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]