MmuINT0179264 @ mm10
Intron Retention
Gene
ENSMUSG00000033961 | Zfp446
Description
zinc finger protein 446 [Source:MGI Symbol;Acc:MGI:2442185]
Coordinates
chr7:12977848-12979378:+
Coord C1 exon
chr7:12977848-12978112
Coord A exon
chr7:12978113-12978997
Coord C2 exon
chr7:12978998-12979378
Length
885 bp
Sequences
Splice sites
5' ss Seq
CTGGTAGGG
5' ss Score
6.35
3' ss Seq
ACATTCTTCCTTTTCTGTAGGTC
3' ss Score
11.22
Exon sequences
Seq C1 exon
GCACATGCGTAAGGAGGCTAACCCACAGTCGGCTCCGTCCTACGTGAGTCCTACGGCTGCGGTTGCAAGAGAAGAACGGCTGCCAAGTCAGGAGTGGGAACCCCCCCCGGAACTCTGCAGCAACGCGACCCCAAGGAGAGGAGCGAAGAGTCTCGCGCGTGAGAGGCCTTGGAAGCGTGCTGTCGTTCTTGCTGAGGCTCACGGGAGACGTAGTCCGAGCGAAGGGGGCCGATCCGGCGGAGCAGATGCAGGATTGGCCGGGCTG
Seq A exon
GTAGGGCAACGCGCGAGGCGGGGCGTTCTCTGAGTGGCAGTGGGACGTGCTCGGTGTCACTTCCTGGAAGTGCCCCAACCTCCTGGGGCTCTCCCACGCGTCAGAGGTGAGCTCACTCCTAGCTGGGCGGGCGCAGCTTCATAATCCGGCTCCTCGGATCACCGAGAGCACCTCCCAGCCGTCGGACCCACCCCTTTCCTGTGTCAGAGCCTGACACCCCAACTCTTGAGTTTCTCCAGAGCAGGACGGATGTCCCAAGAGAGCCGATTTAAAACCAGAATCTTAAGAGCTGGGACACAGGTTCCAGTTTACAACTCCACACAAAACGGGGCTGTTTGATTTTGGCTTTTGTTTCAGGGTTTCACATGCGTAGTAAGCTGAGGCAGTATGAGGCATCCACAATTCAGCTGCGATCAGCGAGCTGTGCCTCCCAACCCATAATAAGCTCCTGAGAATGCACCCAGGCTTTCAGCTCTGCCCCAGGTCTTGTACCTGTGTCTCAGAGCCAACTCTTCAGCTAGCACAGTGGTCTGCCTCCTGTCTGAAGCCTCAGGGCTCCAGGTCACCCAGGATGATGGTCCCACCCCCCTTCCCTCCGTGTCCTGCTTGACCCTTTGTCTACTGGATGAGCCACTTTCATCTCCCAGGAGCCCTGTGTTGTACCCAAATGTTTGAGCCCGTCATTTGCAGTTTTCTAGAAGGTCCCTGGACATTCCACCCAGTCATTTATCTTCAAAACAAAATACAGCTGCTCAACTGCTGTGGGGCTGCACCTCCCATGACCTTCTTTGAAGTTCTCCTGGCCTCAGGGTTCTCTTTTCCGGCTTCTAGGAGGGGTCTGGTGTGTGTCTTCTGACCTTAGATGACATTCTTCCTTTTCTGTAG
Seq C2 exon
GTCCCTCTGTCACCTCCAAAAAGACCCTTCAAAACAAGAATGCCATCTCCTCGGGGTACCCCACACCTATCCCTCAGAGACTCCAGGACCACCCTTGAGGAGCCTGAGGCTGCACGACTCCGTTTCCGGGGTTTCTGCTATGAGGAGGTTGAGGGACCCCGAGAGGCACTGGCCCAGCTTCGAGAGTTGTGTCACCAGTGGCTACAGCCTGAGTCATCCTCCAAAGAACACATAATAGAGTTGCTGATTTTGGAACAGTTCCTGGGTGTGTTGCCCCCTGAGATCCAGGCCTGGGTTCGAGGGCAGCGACCAGGAAGCCCCGAGGAGGCCGCAGTTCTTGTTGAGGGGCTGCAGCATGATCCTGGGCAGCTGCTGGGCTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000033961:ENSMUST00000045810:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.954 A=NA C2=0.322
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0202312=SCAN=WD(100=73.2)
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCACATGCGTAAGGAGGCTAA
R:
CTCTGAGGGATAGGTGTGGGG
Band lengths:
344-1229
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types