MmuINT1004653 @ mm10
Intron Retention
Gene
ENSMUSG00000035697 | Arhgap45
Description
Rho GTPase activating protein 45 [Source:MGI Symbol;Acc:MGI:1917969]
Coordinates
chr10:80016653-80018104:+
Coord C1 exon
chr10:80016653-80016918
Coord A exon
chr10:80016919-80017779
Coord C2 exon
chr10:80017780-80018104
Length
861 bp
Sequences
Splice sites
5' ss Seq
GGGGTGAGT
5' ss Score
7.93
3' ss Seq
TCTTCCCTATGCATACCTAGGAG
3' ss Score
6.38
Exon sequences
Seq C1 exon
AATGAGAGCCAAGCCAGTCACGTGTGGCTCTGACAGCTGGGGAGGGGGTGGCCCTGAGCAATGTGGTCCAGAGACAGCCTGCACCAGGAGTTGCAGGACTGAGACCACCCCCAGACTCCCACCCTGCCGTTGCCCGCCTGTCCTCACCTAGGGCCACTGGGGCTGACTCCACCATGTTCTCTCGGAAGAAGCGAGAACTTATGAAAACTCCCTCTATTTCCAAAAAGAACCGCGCGGGGAGCCCCAACCCGCAGTCCTCCTCAGGG
Seq A exon
GTGAGTGGGGCACAGAGCGGGTGGGGACCCAGGATGACACCCACAGCCTGTGGTCACTGATGGGCATAGCATGCAACTACAACACCATGCTGGGTCCACGCTGGGGGAACCGGGCCAGCTATAGTCCACACCAGGCAGAATGGCCGGCGACAAGGAGTGGCCTGAACCTAGGAGGCCGCATTACGGTAGGTAGGGATGGCCTTGGGTGGCAACCAAGTCACCCATCTTGATGAGGGATGTCCCCGTCGTGTGCCAATGACGGTTGTGGGTAGTGGGTAGAACTGGTATTGGAGTCTAGTGACTACACTGTAATATGGGGTCTGGGCAATTTTAGGGTTCTGAAAGCCAATGGGGAACTGGTGTTGAACTGGGGTGCAGGTGTACAGAGCAGATTGGGGGGGTCTCCAAGTTTCTCCAGGACCTGCTGCTGCCTGCTGAGCTTTTGAGAATTTGTGAGGTTTAGGGGTACCTCTACTAGGTCTGGGGGTGTCCTGAGACACAGGAGCCTGATGATCTTTTCAGGTCACAGAAGTTATCCATGTTTTTAGAAAGTGGACACCCGTAGAGGTATCTGGGGTGGCTACAGGGTTAGAGACTCACAAGAGTCCTCCCCACCCCCACCAGGAAGTGGGGTGCCCCCTCCCTCCTGTGCCCCACAGGCCCCGCCTCTGGCTGTGGTTTGGGTAGGGACGGTTGTTTGTGAAAGCTATAGACAGGAGGATGTTGGGTAACAGGTGGGGGGGGGGGAGCTTAAAATTCTCTGCCCACTGACCTCTGACCCCTGTGAGGACTTTCTGGGGAAACTGAGGCAGTGTCTGGGATGGGGGATCCCCTTGACTCCTCTTCCCTATGCATACCTAG
Seq C2 exon
GAGCTGCCCAGGAAGGATTGGACAGAAGCTCCCGGCCTGGAGCCACCAGCCACATCCCTGTCCACGGTAGCCAAGGGCACAGGGACACTCAAAAGACCCACCAGCCTGAGCCGCCATGCCAGTGCAGCAGGCTTCCCGCTCTCGGGCACTGCCACCTGGACCCTGGGCCGTGGGTACCGAAGCCCCCTTTCTGCGGCCAGTCCGGCAGAGTTACCCACTGAGGGAGCTTTCCCTGATGGCGTAGAAGACATCTCAACCCTGCTGGCGGATGTGGCCCGCTTTGCTGAGGGCTTGGAGAAGCTCAAGGAGTGTGTGCTGCAGGACG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000035697:ENSMUST00000099501:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=NA C2=0.486
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGCTGACTCCACCATGTTCTC
R:
CTACGCCATCAGGGAAAGCTC
Band lengths:
350-1211
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types