RnoEX0064554 @ rn6
Exon Skipping
Gene
ENSRNOG00000001517 | Pdk1
Description
pyruvate dehydrogenase kinase 1 [Source:RGD Symbol;Acc:69427]
Coordinates
chr3:58542018-58545601:+
Coord C1 exon
chr3:58542018-58542107
Coord A exon
chr3:58544151-58544228
Coord C2 exon
chr3:58545525-58545601
Length
78 bp
Sequences
Splice sites
3' ss Seq
TTTTGTTTGATCCACACCAGATG
3' ss Score
6.33
5' ss Seq
ATGGTAAGC
5' ss Score
9.26
Exon sequences
Seq C1 exon
CTTTATTGTTTGGTGGAAAAGGAAGTCCATCTCATCGAAAACACATTGGAAGCATAAATCCAAACTGTGATGTAGTCGAGGTTATTAAAG
Seq A exon
ATGGCTATGAGAATGCGAGACGGCTTTGTGATTTGTATTACATTAACTCTCCTGAACTAGAACTTGAAGAACTAAATG
Seq C2 exon
CGAAATCACCAGGACAGCCAATACAAGTGGTTTATGTTCCGTCCCATCTCTATCACATGGTGTTTGAACTGTTCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000001517-'13-10,'13-9,15-10
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.032 A=0.074 C2=0.000
Domain overlap (PFAM):
C1:
PF104364=BCDHK_Adom3=PD(12.1=64.5)
A:
NO
C2:
PF0251821=HATPase_c=PU(11.2=53.8)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGTTTGGTGGAAAAGGAAGTCCA
R:
TGAACAGTTCAAACACCATGTGA
Band lengths:
159-237
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]