RnoEX0065281 @ rn6
Exon Skipping
Gene
ENSRNOG00000000480 | Phf1
Description
PHD finger protein 1 [Source:RGD Symbol;Acc:1303205]
Coordinates
chr20:5530166-5531939:+
Coord C1 exon
chr20:5530166-5530320
Coord A exon
chr20:5530403-5530560
Coord C2 exon
chr20:5531338-5531939
Length
158 bp
Sequences
Splice sites
3' ss Seq
CTCTTACAGCTTCCCTTCAGGGC
3' ss Score
5.73
5' ss Seq
CAGGTACAA
5' ss Score
7.09
Exon sequences
Seq C1 exon
TTTCATTTCAGGGAGGGAGATTAAAAAGAGGAAATGCCTTTTTGGTCTCCATGCTCGGACCCCTCCTCCTGTGGAGCTTCTCACTGGAGATGGATCCCCCACCAGGTCACTGGTCCAGGGGGGATGGGGGAAATTCTCAAGGATCTGTTTGGAGG
Seq A exon
GGCAGGGCCCTGGGGGAGGGGTCTCACGTCCCCTGGGGAAACGCCGGAAGTCGGAGCCAGAACCCTTGAGGAGGAGGCAGAAGGGGAAAGTGGAGGAACTGGGGCCACCCACAGCAGCTCACAGTCGGCATGGGTCCCAGGAGCAGAGGGCCCTGCAG
Seq C2 exon
GTCACCTCTGGAACTTCACATTGGCTTCCCCACAGACATCCCTAAAAGTTCTCCCCACTCAGTGACTGCCTCGTCTTCCTCGGTCCCAGCCCTGACCCCAGGCCTTTCTAGACATTCACTGCCCCCTTCTCCCTTGTGCCGTAGTTTGTCTCCGGGGACTGGGGGAGGAGTCCGAGGTGGGGTTAGCTACCTGTCCCGGGGTGACCCTGTCAGGGTCCTTGCTCGAAGAGTGCGGCCTGATGGCTCTGTGCAGTACCTAGTCGAGTGGGGAGGAGGGGGCATCTTCTGACAGCTCACTTGTGCTCACCTCCCCATGCACACACTGGCACTTTCACGCCCTGACCTCTGACCTCACCTACAGCTGGGATGTACCTGGAGAGACGGAGTGGTTCTCCCTCCTGCCCAGGCTGGAATCCAGGGGGTTGGAGAAGAGGTCCTCTTCTCTGCCCCTTGATGACTCCTAACCCCCCTCCCATTCCCTTTGATGTTATTTTGTTACA
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000000480_MULTIEX1-1/3=C1-C2
Average complexity
C3*
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.361 A=1.000 C2=0.667
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF140611=Mtf2_C=WD(100=43.3)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTTTGGTCTCCATGCTCGGAC
R:
AGAAGGGGGCAGTGAATGTCT
Band lengths:
246-404
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]