RnoEX6016273 @ rn6
Exon Skipping
Gene
ENSRNOG00000025711 | Spout1
Description
SPOUT domain containing methyltransferase 1 [Source:RGD Symbol;Acc:1563514]
Coordinates
chr3:8748445-8750638:-
Coord C1 exon
chr3:8750593-8750638
Coord A exon
chr3:8750267-8750395
Coord C2 exon
chr3:8748445-8748604
Length
129 bp
Sequences
Splice sites
3' ss Seq
TCCCACCAATTCTTCTCCAGAGA
3' ss Score
7.67
5' ss Seq
AAGGTAAGC
5' ss Score
10.22
Exon sequences
Seq C1 exon
GGTGAACACGGCCAAAGGGTTGAATGGCGAAAATGGAAGCAGCAGA
Seq A exon
AGAAAGAGGAGAAAAAAAAGTGGAAGGACCTCAAGATAATGAAAAAACTGGAGCGGCAGCGTGCCCAAGAGAAACAGGCTAAGCGCCAAGAGGAGGAGGAAGAGGCGGCTGCCCAGAGGAGCAACCAAG
Seq C2 exon
GACGGCCTTACACTCTGAGTGTGGCCCTGCCAGGCTCCATCCTGGACAACGCCCAGTCACCTGAACTCCGCACCTACCTGGCTGGCCAGATCGCCAGAGCCTGCGCCATCTTCTGTGTGGATGAGATTGTGGTGTTCGATGAGGAGGGCCAGGATACCAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000025711-'3-5,'3-2,4-5=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.875 A=0.661 C2=0.056
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0259812=Methyltrn_RNA_3=PU(16.8=90.7)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AACACGGCCAAAGGGTTGAAT
R:
TTGGTATCCTGGCCCTCCTC
Band lengths:
202-331
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]