RnoEX6016656 @ rn6
Exon Skipping
Gene
ENSRNOG00000025498 | Crb2
Description
crumbs 2, cell polarity complex component [Source:RGD Symbol;Acc:1309368]
Coordinates
chr3:22050059-22053796:+
Coord C1 exon
chr3:22050059-22050172
Coord A exon
chr3:22052330-22053202
Coord C2 exon
chr3:22053288-22053796
Length
873 bp
Sequences
Splice sites
3' ss Seq
GGCTGATCTCTCTCCTTTAGGAC
3' ss Score
10.69
5' ss Seq
ATGGTGAGA
5' ss Score
7.23
Exon sequences
Seq C1 exon
GGGATGACTGCAGCGTGGACGTGAATGAGTGTGCCTCAGAGCCATGTCTCAACGGAGGTAGCTGCCAGGACCTGCCCAATGGTTTCCAGTGCTACTGTCAGGATGGATACACAG
Seq A exon
GACTGACATGTCAGGAGGATACGGACGAGTGCCAGTCGGAACCATGCCTGCACGGTGGGACCTGCAGCGACACTGTAGCAGGCTACATCTGCCAGTGCCCCGAGGCCTGGGGTGGACATGACTGTTCTGTGCAGCTCACAGGCTGCCAGGGCCACGCCTGCCCACTGGCTGCCACCTGCATCCCCACCTTCAAGTCTGGTTTCCACAGTTATATCTGCCGTTGCCTGCCTGGGACCTATGGGTCTTTCTGTGACCAGAATACCACTTTCTCGGTTGTGTCTGGGAGTTCCGTGTGGGGATTGGTGCCAGCTGGTGCCTCCCTGGCTTTTGCACTGAGATTTCGTACCACCCTGCTTGCTGGAGCCCTGGCTACTCTTAAGGACTCACGGGACAGCTTGGAGCTGGTTCTGGTGGGGGCCATGCTCCAAGCCACACTCTGGAGACGTGGCACTGCTGACCTTGTCCTTACACTGCCAGACCTAGCCTTAAATGACGGGCATTGGCATCAGGTGGAAGTGACACTCCGCCTGGGAACCCTGGAGCTGCGGCTCTGGCATGAGGGCTGCCCTGGCCAGCTCTGTGTGGTCTCTGGCACTGTGGCTACAGATCCTACAGCCTCGGTGGCTTCTGGGTCTCCGGAGCCCTACTCCATCCATTTGGGCGGCAGGGCCTTTGCAGGCTGCTTCCAGGATGTGCGTGTGGAAGGGCACCTTCTGCTGCCTGAGGAGCTCAAGGGAAGTGTGCTCCTGGGTTGTGAGCGCACAGAGCCTTGCCAACCTCTGCCCTGTGCCAATGGAGGGGCCTGTGTGGACCTGTGGACTGACTTCCGCTGCGACTGCCCGAGACCTTACCATGGGTCCAAGTGCACTGATG
Seq C2 exon
AGGTTCCTGCTGCCACCTTTGGCTTGGGCGGTGTCATGAGCTCAGCCACCTTCCTGCTCCACCAGATGCTAGGCCCGAACCTCACCGTGTCGTTTTTCCTCCGCACTCGTGAGCCCGCCAGCCTGTTGCTCCAGTTTGCCAATGATTCAGTTGCTAGCCTAACTGTGTCCCTGAGTGAGGGCCAGATCCGGGCAGAGGTGCTGGGTCATCCTGCTGTGATCCTCCCTGGGCGCCGGGATGATGGACTCCGTCACTTGGTGGTGCTCAGCTTTGGGCCTGACCAGCTCCAGGACCTGGGCCAGTGGCTGTATGTGGGTGGGAGGCTCTACCCTGATGATACCCAGCCCTGGGGTGGGCCCTTCCGAGGCTGTCTCCAGGACCTACAACTCAACGGCATCCACCTCCCCTTCTTCTCTTCTCCCATGGAGAACTCAAGTTGGCCCAGTGAACTGGAAGCTGGTCAGTCCTCCAACCTCACCCAGGGCTGTGTCTCTGAGGAC
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000025498-'7-6,'7-5,11-6=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.010 C2=0.035
Domain overlap (PFAM):
C1:
PF0000822=EGF=PD(3.8=5.1),PF0000822=EGF=PU(90.3=71.8)
A:
PF0000822=EGF=PD(6.5=0.7),PF0000822=EGF=WD(100=10.6),PF0221019=Laminin_G_2=WD(100=42.5),PF0000822=EGF=WD(100=10.6)
C2:
PF0221019=Laminin_G_2=WD(100=31.8),PF0000822=EGF=PU(3.2=0.6)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCCTCAGAGCCATGTCTCAAC
R:
AAAGCTGAGCACCACCAAGTG
Band lengths:
354-1227
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]