RnoEX6024342 @ rn6
Exon Skipping
Gene
ENSRNOG00000027278 | Pramef8
Description
PRAME family member 8 [Source:RGD Symbol;Acc:1564061]
Coordinates
chr5:162194286-162199050:+
Coord C1 exon
chr5:162194286-162194598
Coord A exon
chr5:162195034-162195621
Coord C2 exon
chr5:162196440-162199050
Length
588 bp
Sequences
Splice sites
3' ss Seq
TCTCCCTGTTTCACTCACAGAAG
3' ss Score
10.02
5' ss Seq
CAGGTAAGG
5' ss Score
11.08
Exon sequences
Seq C1 exon
GTTCCTTCAGACTGAAGATTTGAAGGATGAGTGCCCAGGACTCACCCACACTGCTGAAGCTGGCAGTACTGAGACTACTGAAGGATGAGGCCCTGGCCGTCTCTTCTCTGCAGTACTTGCCCAGGGCTCTCTTCCCACTATTGTTCAAGGAAGCCTTCAATCACAGACAAACTAATGTCCTGAGGGCCATGGTGGCAGTGTGGCCCTTTCCCTTTCTCCCTCTGGGGACCCTGATGAAGACTCCCCACCTGGAGATCTTGCAGGCTGTGCTGGATGGAGTAGACATGCTGTGGACACAGAAGGTTCTTCCCAG
Seq A exon
AAGGCGGAAGCTTCAAGTGCTGGACCTGAGGAATGTGCACCATGACTTCTGGGATGTTTGGGTTGGACCAGAGGATGGAGGCCGCTCGGCAGAGGCTGTGTGTGAAGAGCAGACAGCGAAGCGCCTTCACAGATACGTACTGAGGCGGCGTTTGAAGGTGGTCACTGACCTCTGCCTCAGGTTCCACCTGAACGAGCACCAAGCATACCTGTTGCAGTGGGCCCAGCAGAGAAGAAGTTCCATTCGGCTGTGCTGTGTGAAGATGCAGATTTGGGCTTTGCCTGCGTACACCGTCCGGAAGGTCTTGATGGTGTTCCAGCCAGACAGCATCCAGGAGTTGGAACTCAATACAGGTTGGAGTCTCTCCACTCTGGTGCATTTTGCATCTTACCTGGACCAGATGAGAAACCTACAGAAGCTCCTTATAACACGGATTCACAAGAACACCTTCAAGGTACTAAATACCTCCTCGGACATACAGAAGTGTATCACCAAGTTTGTTTCTCAGTTCTCCAAACTCAACTCTCTCCAGCATCTCTCCATGAACGGCATCTACTTTTCCAGTGAGCACATGAAACAGTTGTTCAG
Seq C2 exon
GTACCTGAAGACCCCTTTGGAGACCCTGTCCATCACAATGTGCAAGCTTTCACATTTAGACTTGAGTTCCTTGTCCCAGAGTCAGAGCCTCCATCAACTCAAACACCTGAATCTGAGGCTTGTGAAATTAATTGACTTACGTCCTGTGCCTTTCCATGATCTCCTAAGGAGTGTTGCAGACACTCTGCAGACCCTAGAATTGGAGGGCTGTTGGATGGTAGACTCGCAGCTCATTGCCCTCCTGCCTGCCCTGAGCCAGTGCTCCCAGCTCACCAGGGTCAATTTCTATGACAATGACATCTCCATGGCTGTTCTGAAGGACCTTCTGTATCATACAGCCAATCTGAGCCAGCTTACCCAGGAGCTGTATCCTGCCCCTTTGGAGTGCTATGATGACACAGGTGATGTCCTTATGGGAAGATTTGTCCAGCTTTGTCCTGAGCTCATGGAGACACTTATGACTATAAGGCAGCCCAAGAGAGTCTCCTTTGCTACGTATA
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000027278-'5-4,'5-3,8-4=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.005 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTGAAGCTGGCAGTACTGAG
R:
TGATGGAGGCTCTGACTCTGG
Band lengths:
357-945
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]