RnoINT0044374 @ rn6
Intron Retention
Gene
ENSRNOG00000032805 | Cyp2f4
Description
cytochrome P450, family 2, subfamily f, polypeptide 4 [Source:RGD Symbol;Acc:2476]
Coordinates
chr1:83943115-83943790:+
Coord C1 exon
chr1:83943115-83943291
Coord A exon
chr1:83943292-83943648
Coord C2 exon
chr1:83943649-83943790
Length
357 bp
Sequences
Splice sites
5' ss Seq
CAGGTAGGC
5' ss Score
9.88
3' ss Seq
GGACCGTCTGTCAACCACAGGAG
3' ss Score
6.2
Exon sequences
Seq C1 exon
ATGTACAACATCTTCCCGAGTCTCCTGGACTGGGTGCCTGGGCCGCACAGACGCGTGTTCCGGAACTTTGGGGGCATGAAAGATCTCATCGCCCGCAGCGTCCGCGAGCACCAGGACTCCCTGGACCCCAACTCTCCCCGGGACTTCATCGACTGCTTCCTCACAAAAATGGTACAG
Seq A exon
GTAGGCATGAGATGAAGATTCTCCCTCTGCGCTGCTGTGAAGTCCTCCTGCTGCCCCCAGCCTCAAACATGCAGGGGTCTCAGCTCGGGTAGAGTCGTGACGCTGTGGCACCTGGCCCTGCTGAACGCCTGCCCAGAGAGACTACCAATTAACAAGTCATTAAGCCGTTGTTGTGGATGGTCAAGTTGTTCAACTTGGGCCTTGACACTTTTTATCTCTCCCTTCACTCTCCTCGGCTCCCACAAAAAAGGTAGCTCAAGAGAGGGACAGAACCAGCGACTAGTAATGGCAGCACACAGGCCCCAAAGTCTAAGCTTCTCCCAGTCCCTGACTCACAGGACCGTCTGTCAACCACAG
Seq C2 exon
GAGAAGCAAGACCCACTGAGCCACTTCAATATGGACACCCTACTGATGACCACACACAACCTGCTCTTTGGTGGAACGGAGACTGTGGGCACCACTTTACGCCATGCCTTCCTCATTCTTATGAAGTACCCCAAAGTGCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000032805:ENSRNOT00000068690:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.017 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006717=p450=FE(12.7=100)
A:
NA
C2:
PF0006717=p450=FE(10.3=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGTACAACATCTTCCCGAGTCT
R:
CTTGCACTTTGGGGTACTTCA
Band lengths:
318-675
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]