RnoINT0070975 @ rn6
Intron Retention
Gene
ENSRNOG00000006116 | Hk2
Description
hexokinase 2 [Source:RGD Symbol;Acc:2797]
Coordinates
chr4:113571727-113573301:-
Coord C1 exon
chr4:113573146-113573301
Coord A exon
chr4:113571961-113573145
Coord C2 exon
chr4:113571727-113571960
Length
1185 bp
Sequences
Splice sites
5' ss Seq
AGAGTAAGC
5' ss Score
6.31
3' ss Seq
ACACCCTGTCTGTTTCCCAGGGA
3' ss Score
9.28
Exon sequences
Seq C1 exon
GTTTGAGAAGATGATTAGCGGGATGTACATGGGGGAGCTGGTCAGGCTCATCCTGGTGAAGATGGCCAAGGCAGAGCTGTTGTTCCAAGGGAAACTCAGCCCAGAACTCCTTACCACTGGCTCCTTCGAGACCAAAGATGTCTCGGATATTGAAGA
Seq A exon
GTAAGCTTCTAAGTCTCCATGAGCAGGGCAGGAACCGGGGAGGGAAATGAGGGGCTTGGTTTGGAGTTCTGTGTGTGCCTCTGTGTGCCTGTGTATTCGTGTGTGGGTATGTGTTCTGCCTTTTTTTTTTTTTTTCTCTTAGATGTATCTCTTTCATGCAGCTGTCTTCAGACACACCAGAAGAGGGCATCAGATCCCATTGCAGATGGTTGTGAGCCACCATGTGGTTGCTGGGAATTGAACTCCCGTTGGTGCTCTTAACCTGTGAGCCATCTCTCCAGCCCTGCAATGTCTACCTTGAAGACAGACTTTCTCACTAGCCTGGAGTTTACTAAGGAAGCTAGGCTAACTGACCAATGAGCCTCCAAGGATCTACTGGTCCCCACCTCCCCAGTGCTCTGGGATTAGAGACAAACCACATCTGAATCTTTTTTATATAGCATAAATTGTGGGGATTGAACTCAGGTTCTTGTGCTTGCCAACGACATCACCAAACAGTGTTGCAAAGATTTAGGTCTGCTGTTCTGAGCTTCAGATTTCTACTTGATTGAGGGGGTCTTAGAGTTTCAGGGACGTGATGGAGAGAGGAAGTAGTTTTAGGCAAAGAAGGCCAATGAAATAGTTCAAAGGACTTTGCTAACGAGGACCCTTGGAGCAGTTCTGCCTGCTCTTTGTTAAGCCCAAAGGGAGATGAAGGCCCGTGTTATAGAGGCATGGGGCTCCTCAAGGAGAGGCTCAGGCCCTACCTCCAGCCCTTTCCCAATGTCTTCAGACAGACTGGCTTTTGCCTTTCTAGAGTGCAGCAGGTGGGGCTCACACTGAATTGAAGTACTCAGTGGTGGCCGGCAGCTGAGTTGCAGTGAGCAGGAACCCACACCCACACGTTCTCTTACTCAGCATGTGCAGCAGGGAAAACAGGGTCTTGTCTTTCTAAGGTACACACAAAGGAACTGCAAGGTTTTCCTTGTGGGAAACAAGAGCACCTACAGAAACCAGACCTCCCTCTGTGTGCGTATGCCTGTGTGCGTGTGTGGGTGGTCTGGGAGAGGAGCCTTGAATTACTGTGTAGTCAGCTCTCCCTGGAAGGATGACTTTGCTAGAACCGAGGGACTCCCTTGCCAGCCCACAGCCCCTCCCACTGGCCACAGTGAATTCAGCATGGCTCACACCCTGTCTGTTTCCCAG
Seq C2 exon
GGATAAGGATGGAATCGAGAAGGCCTACCAAATCCTGATGCGCCTGGGTCTGAATCCATTGCAGGAGGATTGTGTGGCCACGCACCGAATCTGCCAGATTGTGTCCACGCGCTCGGCCAGTCTGTGCGCAGCCACCCTGGCCGCGGTGCTGTGGCGAATCAAAGAGAACAAGGGCGAGGAGCGACTTCGCTCCACCATCGGTGTCGATGGCTCCGTCTACAAGAAACATCCCCA
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000006116:ENSRNOT00000008813:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.019 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0372711=Hexokinase_2=FE(21.7=100)
A:
NA
C2:
PF0372711=Hexokinase_2=FE(32.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGTACATGGGGGAGCTGGTC
R:
TTGTAGACGGAGCCATCGACA
Band lengths:
356-1541
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]