RnoINT0096276 @ rn6
Intron Retention
Gene
ENSRNOG00000049695 | Myh1
Description
myosin heavy chain 1 [Source:RGD Symbol;Acc:735061]
Coordinates
chr10:53725808-53727433:+
Coord C1 exon
chr10:53725808-53725944
Coord A exon
chr10:53725945-53727177
Coord C2 exon
chr10:53727178-53727433
Length
1233 bp
Sequences
Splice sites
5' ss Seq
AAGGTATGA
5' ss Score
9.11
3' ss Seq
TAGCAACCACTGCATTTCAGAGA
3' ss Score
2.15
Exon sequences
Seq C1 exon
GTCTTTTTCAAAGCTGGTCTTCTGGGGCTCCTAGAGGAGATGAGAGATGACAAGTTGGCCCAGCTGATTACCCGGACGCAGGCCATGTGCAGAGGGTTCTTGGCAAGAGTGGAGTACCAGAAGATGGTGGAGCGAAG
Seq A exon
GTATGAAAATTTATAGTTTATTCACATCTCGTGTCTTCAAAAAGACACTCAAGGTGGCCAACAGTTAGGACTTGGTTCTGTTAAGAAGATCAAAGTAAGAAGGAATAAGTTGACTTCTGTGGGAAAGTGGGAGAACCTAAGTTCTGTCAGCATGAAATATGTCCTGAGTGTCTGGGTAAAACTTGAGAAGCATCAGGAATGGATAGTTCTGTATCTAACACTAGACATATACCAGTGAGTTGGCAGAGATAAAGTTTTTCCCTTTTGTTTCTTTTTGATGAGGTTTTACACAGAGGACCTTAAAATGGCTTTATATAGTATATAAAACTATTTCTTCAGTTGGATTATGTCTATTATTGAACTAACAATTTAAAATAGGGTACCTGTGAAGAGCTTTTCACAATAGGTTGCTACAATGTCATCAAGGGAGTTAACTTCCTGGTAGTCTAGTTGAACAACTGACACATGACAGAGCCTGATGTCTTGGGGCCAGAAATGAGAAGTGCATTACACATGGCCAACTGTGCATCCACACACATGAACAAGTCTGTGTACACAAAAAGGTGGCATGAGACAACTTTTATCACTTGAAGTTGTGCTTAATTTTTCTGACCATAGTCACTATGTTATATTTGGGTAGCCAACTACCTATATATCATGGTCAATAATTTTTAAATAAACCAGATTAAGGCAAAAGTAACATTGTAGAGTAGAATGCTGCACTCATGCAACATTAAAATGTAAGACTGGAGATTGTCAAAAATCTGACTTTTAAAGTCTCCCACACCACCTCTTTCATAGACCATGGGCCCTTCCACCTCCCTGTGCTACTTTCCTGGGAGTGTTTGAAGCACTGATGAACAGCAGTGTCTCTCAAAGGGCAATCCTCAATTTGTGTTATTGATAACTTTTAGGATCTTATTAAAATGCAAAAAGTCAGGTCTGTGTCAAAGACTCTGAATCAGAATCTCTGGAAGTGAAATTCAAGAGTCAGTATTTTCAATAAGCTCTTCAGGTGATCTCAACCATGTCCAAGGTTGGGACACAATGGTGTAAAGAAATAAATAGGAGTCCTTGAGCATCGCCATGAAATAAAACTTTTCTAATCCACAAGGTTGAATGCAAATAAAGAATGGGGTAGGATGGAGGGGGACAGAAGCTCTGACCCTCACTCTTGAAATTTACGCTTGCTTCATCATCCACTGTATCTAATTAGCAACCACTGCATTTCAG
Seq C2 exon
AGAGTCCATCTTCTGCATCCAGTACAACATCCGCGCCTTCATGAACGTCAAGCACTGGCCCTGGATGAAACTCTTCTTCAAGATCAAACCACTGCTGAAGAGCGCTGAGACCGAGAAGGAGATGGCCACCATGAAGGAGGAGTTCCAGAAAACCAAGGATGAACTTGCCAAATCGGAGGCAAAGAGGAAGGAACTTGAAGAAAAGATGGTGTCCCTGTTGAAAGAAAAAAATGACTTGCAACTCCAAGTTCAGGCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000049695:ENSRNOT00000004236:19
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.140
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=PD(1.5=6.5),PF0061222=IQ=WD(100=43.5)
A:
NA
C2:
PF0157614=Myosin_tail_1=PU(28.0=61.6)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg19)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GACAAGTTGGCCCAGCTGATT
R:
AGCCTGAACTTGGAGTTGCAA
Band lengths:
345-1578
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]