RnoINT0131939 @ rn6
Intron Retention
Gene
ENSRNOG00000000500 | Scube3
Description
signal peptide, CUB domain and EGF like domain containing 3 [Source:RGD Symbol;Acc:1304880]
Coordinates
chr20:7740815-7741350:+
Coord C1 exon
chr20:7740815-7740976
Coord A exon
chr20:7740977-7741182
Coord C2 exon
chr20:7741183-7741350
Length
206 bp
Sequences
Splice sites
5' ss Seq
CAGGTGCCA
5' ss Score
3.75
3' ss Seq
GGGACCTTGCTTCACTGCAGGTC
3' ss Score
7.23
Exon sequences
Seq C1 exon
TCAGCTGCCCGCAGGGAACGTATTACCACGGCCAGACGGAGCAGTGTGTGCCATGCCCAGCGGGCACCTTCCAGGAGAGGGAAGGGCAGCTCTCCTGCGACCTTTGCCCTGGGAGTGATGCCCACGGGCCTCTTGGAGCCACCAACGTCACCACGTGTGCAG
Seq A exon
GTGCCAGGGGAACAAACAACACAGAATGGGCGAGGGTGGGCACTGGGATGCCCATGGGCATGGGATTTCCCAAAGGGCAGGCCCAGGCTCCAGGCCAGTGTCCTAGTTGATGTCCTGTTTGTAAGCCTCTGCTCCCTAGGACTGGGTGATCCCATGGAGTGAGTCAGGGTCAACCCCATCAGAGCTGGGACCTTGCTTCACTGCAG
Seq C2 exon
GTCAGTGCCCACCTGGTCAACACTCAGGGGATGGATTTAAGCCCTGCCAGCCCTGCCCACGTGGCACCTATCAGCCCGAAGCAGGACGAACCCTGTGCTTCCCCTGTGGCGGAGGCCTCACTACCAAGCACGAGGGGGCAGTCTCCTTCCAGGACTGTGACACCAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000000500:ENSRNOT00000000593:16
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF076998=GCC2_GCC3=WD(100=87.3)
A:
NA
C2:
PF076998=GCC2_GCC3=WD(100=84.2)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TATTACCACGGCCAGACGGAG
R:
GGTGTCACAGTCCTGGAAGGA
Band lengths:
306-512
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]