RnoINT0142827 @ rn6
Intron Retention
Gene
ENSRNOG00000025711 | Spout1
Description
SPOUT domain containing methyltransferase 1 [Source:RGD Symbol;Acc:1563514]
Coordinates
chr3:8750593-8751324:-
Coord C1 exon
chr3:8751093-8751324
Coord A exon
chr3:8750639-8751092
Coord C2 exon
chr3:8750593-8750638
Length
454 bp
Sequences
Splice sites
5' ss Seq
CCGGTAGGT
5' ss Score
9.39
3' ss Seq
CCTTCCTTCCTTCCTTTCAGGGT
3' ss Score
13.68
Exon sequences
Seq C1 exon
TGCTGTGAACTCGTTGCCCTGGAGACAGGACACCAGAGCTGCAGCCTCCGAACAGGCAGGTGGAGTTTCCGAGCTCCACCTCAGCCTGGCTCCGAGGATACGCCCCACGTTCGGCTGAGTCTCTGATTGGGTAGCTCTGCGGAGGTGTGGCCGGAAGAGGCGTGACTATTGGCAGGCGCGTGTTTTTCCTGGCAACATGGCGGAGCGCCCGGGGAAGCGTCCCTGTGGCCCG
Seq A exon
GTAGGTGGCGAACGGATGGGTTCCGGGGTAGTCCCGGGAGCTGCAGCGTCTCACAGTGGTGTCGGCCTGGGTCTTCTGCTACCTCCTCGCTCCTGGTCCGAGGCCTGAGCTTGTGGGGTTAGTTGATCCGATCTACATTCATCCGCAGGGCGACGGTGTCCTGAGTCCTCTCCTGGCTTGTTTTCTGATTTCTAGGGCGGCTTTATGGACAGACCTGTGTTCCCCTCCCCTCAGTTTCTTCTTTTGCCCCATGGAAATTCATTCCTCAACTTCCTGGTTGCTTACTGTGCCGGACCTGATCCCAGGCATTGTGCACAGCGAGTCCCTGGGTACTTCCTCCACCCTGCCTGATTTTGAGAGAGTCAGATGGTGGGAGCCTCGCGGGGTTGCACCTCTCTTATATGTGGCAGTTCAGCCTCTCATTCTTCCTCCTTCCTTCCTTCCTTCCTTTCAG
Seq C2 exon
GGTGAACACGGCCAAAGGGTTGAATGGCGAAAATGGAAGCAGCAGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000025711:ENSRNOT00000030623:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=NA C2=0.875
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCTGTGAACTCGTTGCCC
R:
TCTGCTGCTTCCATTTTCGCC
Band lengths:
278-732
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]