RnoINT0161646 @ rn6
Intron Retention
Gene
ENSRNOG00000011294 | Usp33
Description
ubiquitin specific peptidase 33 [Source:RGD Symbol;Acc:1307848]
Coordinates
chr2:257584403-257585916:+
Coord C1 exon
chr2:257584403-257584515
Coord A exon
chr2:257584516-257585746
Coord C2 exon
chr2:257585747-257585916
Length
1231 bp
Sequences
Splice sites
5' ss Seq
AGGGTGAGT
5' ss Score
9.25
3' ss Seq
TTAAAACCAAACACTTGCAGGTG
3' ss Score
5.18
Exon sequences
Seq C1 exon
CTCAGTCTTCATCTCCAAAGAGGAAAAAGCAGCATAAGAAGTACAGAAGTGTCATCTCTGACATATTTGATGGGACAATCATTAGTTCAGTACAGTGTCTGACGTGTGATAGG
Seq A exon
GTGAGTATGGAGAACCGTAACAGGATGTTTCTGTCCAGTGTTATTTGTAAAAACCTAAGGATGGGAAGAGTTAAATAGATCATTGCTGTTGCAGCCCACACATTTCTTGTGTGTCTGTTAGTTATACATTGAGTTGAGATGTAGAGTAACAGTACAGCCCACATCCTTTGTGCAGGACCACGGGCTCTTTGTCTGTTTTGGTTACATATAAAGGTGAGAAGTTACTTGGCAACAAGAATTGTTAACAGTGCTTCTTCTCCCACGGCTGATGAAATTAGGTATGCCCAGAAACACTTGCATTCACAAGCAAATGGCAGTTGAGTGTATTCTTACATTTGCCTTTGCAGGTGGCTTGTAAATGATACATGTGTTTCAGTGATGATACCAAAGTCACAAACACGGTTTTGTCTCAAACAAATTTCATGAGGGTTGACTAACACCCAATCCTTCAGAAAGAAAGAAATCCTTCAGAAAAGAAAAAATTAAGATTTCTTAATTTTTTCATTTTAGTGAAATACAGTAATTAGTATGCTTAGTGAATGAGAACTCATGTCTAAGAAACGAGAAAATCAACTTTCAATTATTGTGTTATAATGTTAAGTCACTCAGTAGGCACTCAGTAGGGTCATTTTCATACTGATGGTGTATTATCCCGAAACGTTTTATCTAGTGTATGTGTATTGCTGTAGTTAAGGACAGACTGCATGTGACTGTCATGGAAGATTCTGTTAGCAGAGTATTGTAATCATCTTAGTGTGGTATTTACTCCATGGTTGCCTGAAGCAGCGTATCTCCCATGATGCATCCCTGTTGTTAAGTGCTGGTTGATGCCAGATCTGGTTTTCTCCTTACCACTCTACCACAGCCTGCAGTTTCATGTACTTTGGAGGCTGAGGGGAGAGGATGCCTTGAGAACAGTTTGGATGGTACAGTGTATAATAAGACCTAGTCCCTCAACACTCCTAGAAAGGAGCGTGAATGTCAGTCTCCATTGCCCGTAGCTGGGTTTGTTGTGCTTCTCTGAGCCATAGAAATACTCATGACTCGTAGGAAGATTATATTTGGAACAGTACCTGGTACAAAGTGGACTCATTTCTATGTGTAGTATTCTAAAGCTGTTGGTATTTATATTTGCTTATAGTAATAGCTTGCTTTCATATGTGGAAGAAATCTAACCCTGTAAATAACTGTCCTGGATTCTTTCCATCCTGTTAAAACCAAACACTTGCAG
Seq C2 exon
GTGTCTGTAACCCTCGAGACCTTTCAGGATCTGTCCTTGCCGATTCCTGGCAAGGAAGACCTGGCTAAGCTGCACTCCTCCAGTCACCCAACTATAGTCAAAGCAGGGTCGTGTGGCGAAGCCTACGCTCCACAGGGGTGGATAGCTTTCTTCATGGAGTACGTGAAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000011294:ENSRNOT00000066125:11
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.289 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0044324=UCH=FE(7.0=100)
A:
NA
C2:
PF0044324=UCH=FE(10.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCAGTCTTCATCTCCAAAGAGGA
R:
TCACGTACTCCATGAAGAAAGCT
Band lengths:
278-1509
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]