BtaEX0034193 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000032544 | SPAG1
Description
sperm associated antigen 1 [Source:HGNC Symbol;Acc:HGNC:11212]
Coordinates
chr14:66321916-66331005:-
Coord C1 exon
chr14:66330873-66331005
Coord A exon
chr14:66328200-66328326
Coord C2 exon
chr14:66321916-66322080
Length
127 bp
Sequences
Splice sites
3' ss Seq
GTTCCATTTCATTACACCAGAGC
3' ss Score
5.56
5' ss Seq
AAGGTGAGG
5' ss Score
9.16
Exon sequences
Seq C1 exon
ATGAAAACGTATTTAAAACCCTTAAAGAAGAAGGAAATCAATGTGTAAAAGACAAAAACTATAAAGATGCCCTTAGTAAATACAGTGAATGCTTGAAGATTAACAACAAGGAATGTGCCATTTATACAAACAG
Seq A exon
AGCCCTCTGTTACTTGAAGCTGGGCCAATTTGAAGAGGCAAAGCAGGACTGTGAACAGGCCCTTCAGATGGATCACGGGAATGTGAAAGCTTGCTATAGACGAGCCCTTGCTCACAAAGGGCTCAAG
Seq C2 exon
AATTATCAGAAAAGTTTAAACGATCTCAATAAAGTTCTCCTGCTAGACCCAAGTATTGTTGAGGCAAAAATGGAACTGGAAGAGGTAACCAGAATCCTTAATATTAAGGATAATACAGCATCATTCAACAAAGAAAAGGAGAGAAGGAAAATTGAGATTCAAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000032544_MULTIEX1-2/2=1-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.067 A=0.000 C2=0.127
Domain overlap (PFAM):
C1:
PF134141=TPR_11=PU(60.9=93.3)
A:
PF134141=TPR_11=PD(37.7=59.1),PF0051523=TPR_1=PU(41.2=31.8)
C2:
PF0051523=TPR_1=PD(52.9=32.7)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCCTTAAAGAAGAAGGAAATCAATGT
R:
TCCTTTTCTTTGTTGAATGATGCTGT
Band lengths:
255-382
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development