GgaEX1054861 @ galGal4
Exon Skipping
Gene
ENSGALG00000016037 | SPAG1
Description
sperm associated antigen 1 [Source:HGNC Symbol;Acc:HGNC:11212]
Coordinates
chr2:128202726-128206313:+
Coord C1 exon
chr2:128202726-128202867
Coord A exon
chr2:128203801-128203927
Coord C2 exon
chr2:128206149-128206313
Length
127 bp
Sequences
Splice sites
3' ss Seq
ATTCTGTTTTTCTGTGTTAGAGC
3' ss Score
7.84
5' ss Seq
CAGGTGAGG
5' ss Score
10.07
Exon sequences
Seq C1 exon
GTGAGAAAGCTGAGGAAAAGTTCAGGACATTAAAAAATGAAGGGAATGATTTTGTGAAGAAGGGTAAATATGATGAAGCTGTAAATAAATACAGTGAATGCTTGAAGTTAAATACCAAGGACTGCACAATATACACTAACAG
Seq A exon
AGCTCTCTGTTACTTGAAACTGCATAAATATGAAGAGGCTAAGCAAGATTGTGATCATGTTCTTCAAATAGAAGATTGTAATATTAAAGCTTTTTACAGAAGAGCACTAGCATACAAAGGATTACAG
Seq C2 exon
AGCTATCAAGCCAGTGTTGATGATCTCAAGAAAGTACTTCTAATAGATCCAAATGTTCTTGAAGCAAAAAAGGAACTGGAGGAGATAACCCAACTGCTCAGCCTTGGCGGCGTTGCTGTTGCTGACTGTCAACAAAAGCAAAGGAAGAAAATAACCATACAAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000016037_MULTIEX1-5/5=4-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.125 A=0.000 C2=0.018
Domain overlap (PFAM):
C1:
PF134141=TPR_11=PU(60.0=87.5)
A:
PF134141=TPR_11=PD(38.6=60.0),PF0051523=TPR_1=PU(41.2=31.1)
C2:
PF0051523=TPR_1=PD(52.9=32.7)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAGCTGAGGAAAAGTTCAGGACA
R:
CAAGGCTGAGCAGTTGGGTTA
Band lengths:
242-369
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]