BtaEX6008998 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000020984 | RAPGEF4
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:HGNC Symbol;Acc:HGNC:16626]
Coordinates
chr2:23738341-23743001:-
Coord C1 exon
chr2:23742948-23743001
Coord A exon
chr2:23742526-23742632
Coord C2 exon
chr2:23738341-23738462
Length
107 bp
Sequences
Splice sites
3' ss Seq
CTCACTTGATGAAAATTCAGGTT
3' ss Score
1.73
5' ss Seq
CAGGTATGT
5' ss Score
9.8
Exon sequences
Seq C1 exon
ACACCTCTCATTGAACCTCACGTTCCTCTTCGTCCTGCTAACACCATTACCAAG
Seq A exon
GTTCCTTCAGAGAAGATCCTCAGGGCTGGAAAAATTTTACGAAACGCCATTCTCTCTCGAGCACCTCACATGATAAGAGATAGAAAATACCACCTAAAGACATACAG
Seq C2 exon
ACAGTGCTGTGTGGGGACCGAGCTGGTGGACTGGATGCTGCAGCAGACTCCATGTGTTCACTCCCGAACGCAAGCTGTGGGCATGTGGCAAGTCCTGGTAGAAGACAGCGTTCTCAACCATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000020984-'19-17,'19-15,20-17=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.444 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0061016=DEP=PU(19.7=38.9)
C2:
PF0061016=DEP=FE(57.7=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CACCTCTCATTGAACCTCACGT
R:
TGGTTGAGAACGCTGTCTTCT
Band lengths:
173-280
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]