DreEX0060854 @ danRer10
Exon Skipping
Gene
ENSDARG00000079872 | rapgef4
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:ZFIN;Acc:ZDB-GENE-070912-385]
Coordinates
chr9:2975734-2983874:-
Coord C1 exon
chr9:2983810-2983874
Coord A exon
chr9:2977464-2977570
Coord C2 exon
chr9:2975734-2975855
Length
107 bp
Sequences
Splice sites
3' ss Seq
AGTGTATATTTGTCTCTCAGGTG
3' ss Score
7.82
5' ss Seq
CAGGCGAGT
5' ss Score
2.92
Exon sequences
Seq C1 exon
GAATGCCTGACAAAGAGAATCTGAGCAACAGTTCTCCTGGACTATCACCCAAGCACTTAAATAAG
Seq A exon
GTGCCGTCAGAGAAGATACTTCGGGCAGGAAAAGTGCTACGAAACATCATTTTATCTCGAGCGCCTCATATGATAAGAGACAGGAAATACCACCTGAAGACGTACAG
Seq C2 exon
GCGGAGCTGCATCGGCACTGAGCTCGTTGATTGGCTGTTGCAGCACACATCATGTGTTCACTCTCGTGTTCAGGCTGTAGGGATGTGGCAGGTGCTGCTGGAGGAGGGAGTGCTTAACCACG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079872-'8-9,'8-8,10-9
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.848 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0061016=DEP=PU(19.7=38.9)
C2:
PF0061016=DEP=FE(57.7=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AATGCCTGACAAAGAGAATCTGAGC
R:
CGTGGTTAAGCACTCCCTCCT
Band lengths:
186-293
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]