BtaEX6009014 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000020984 | RAPGEF4
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:HGNC Symbol;Acc:HGNC:16626]
Coordinates
chr2:23670249-23677183:-
Coord C1 exon
chr2:23677086-23677183
Coord A exon
chr2:23670841-23670966
Coord C2 exon
chr2:23670249-23670427
Length
126 bp
Sequences
Splice sites
3' ss Seq
TTCCATATTATTATTCACAGACT
3' ss Score
5.44
5' ss Seq
GAGGTAAGG
5' ss Score
10.28
Exon sequences
Seq C1 exon
AAAAGGTGGTGCTCAAACCTAATGATGTTTCAGTATTTACAACGCTCACCATTAATGGACGTCTGTTTGCTTGCCCGCGAGAGCAATTCGATTCACTG
Seq A exon
ACTCCCTTGCCAGAACAGGAGGGCCCAACTGTTGGAACAATGGGAACTTTCGAACTGATGAGCTCCAAAGATTTGGCCTACCAGATGACAATTTATGATTGGGAGCTCTTCAACTGTGTGCATGAG
Seq C2 exon
CTGGAGCTAATCTATCACACATTTGGAAGGCATCATTTTAAAAAGACCACAGCAAACTTGGATTTGTTCCTGAGGAGGTTTAATGAAATTCAGTTTTGGGTTGTCACTGAGATCTGCCTTTGTTCCCAGCCTAGCAAGCGTGTTCAGCTCTTAAAAAAATTTATTAAGATCGCAGCCCA
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000020984-'39-37,'39-36,40-37=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.024 C2=0.000
Domain overlap (PFAM):
C1:
PF0078818=RA=PD(35.0=84.8)
A:
PF0061714=RasGEF=PU(13.0=57.1)
C2:
PF0061714=RasGEF=FE(31.9=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGTGGTGCTCAAACCTAATGA
R:
AGAGCTGAACACGCTTGCTAG
Band lengths:
246-372
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]