MmuEX6102141 @ mm10
Exon Skipping
Gene
ENSMUSG00000049044 | Rapgef4
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:MGI Symbol;Acc:MGI:1917723]
Coordinates
chr2:72229006-72234786:+
Coord C1 exon
chr2:72229006-72229103
Coord A exon
chr2:72234077-72234202
Coord C2 exon
chr2:72234608-72234786
Length
126 bp
Sequences
Splice sites
3' ss Seq
TTTTTCACAATCTTTCCCAGACT
3' ss Score
7.22
5' ss Seq
GAGGTAAGA
5' ss Score
10.06
Exon sequences
Seq C1 exon
AAAAGGTGGTGCTGAAATCTAATGATGTTTCAGTATTTACGACGCTCACCATTAATGGACGCCTGTTTGCCTGCCCGAGAGAGCAATTCGACTCACTG
Seq A exon
ACTCCCTTGCCGGAACAGGAAGGCCCGACCACTGGGACAGTGGGAACATTTGAGCTGATGAGCTCGAAAGACCTGGCGTACCAGATGACAACCTACGATTGGGAACTCTTCAACTGTGTGCATGAG
Seq C2 exon
CTGGAGCTAATCTACCACACATTTGGAAGGCATAATTTTAAAAAGACCACGGCAAACTTGGATTTGTTCCTGAGGAGGTTTAATGAAATTCAGTTTTGGGTTGTCACTGAGGTCTGCCTTTGTTCCCAGCTCAGCAAACGTGTTCAGCTTTTGAAAAAATTTATCAAGATAGCGGCTCA
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000049044-'65-51,'65-49,74-51=AN
Average complexity
A_S
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.190 C2=0.000
Domain overlap (PFAM):
C1:
PF0078818=RA=PD(35.0=84.8)
A:
PF0061714=RasGEF=PU(13.0=57.1)
C2:
PF0061714=RasGEF=FE(31.9=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGTGGTGCTGAAATCTAATGATGT
R:
CTGAACACGTTTGCTGAGCTG
Band lengths:
242-368
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types