DreEX6034779 @ danRer10
Exon Skipping
Gene
ENSDARG00000079872 | rapgef4
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:ZFIN;Acc:ZDB-GENE-070912-385]
Coordinates
chr9:2952281-2952970:-
Coord C1 exon
chr9:2952873-2952970
Coord A exon
chr9:2952636-2952761
Coord C2 exon
chr9:2952281-2952459
Length
126 bp
Sequences
Splice sites
3' ss Seq
TTAATGGGCTGTTGGTGTAGACG
3' ss Score
3.35
5' ss Seq
GAGGTACAA
5' ss Score
5.13
Exon sequences
Seq C1 exon
ATAAAGAGCTTCTGAAGCCGTCTGATGTTTCCATCTTCTCATCTCTCAGCATTAATGGCCGTCTGTTCGTCTGCCCGAGGGACCAAATCGACTCTTTG
Seq A exon
ACGCCGCTGCCCGAGCAGGAGGGACCGTCTGCAGGCTCCATGTCCACGTTTGAGCTCATGAGCTCTAAAGATCTGGCCCATCAGATGACCCTCTACGACTGGGAGCTCTTCCACTGTGTGCATGAG
Seq C2 exon
CATGAGCTGATCTACCACACGTTCGGCCGACATCATTTTAAGAAGACCACCGCCAATCTGGACCTGTTCCTGCGGCGCTTCAATGAGGTGCAGCTGTGGGTGGTGACGGAGGTCTGTCTGTGTCCCGCGCTCAGCAAACGAGTGCAGCTCCTCAAGAAGTTCATCAAGATCGCTGCACA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079872-'30-33,'30-32,31-33=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.020 A=0.159 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
PF0061714=RasGEF=PU(13.0=57.1)
C2:
PF0061714=RasGEF=FE(31.9=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGAAGCCGTCTGATGTTTCCA
R:
ACTTCTTGAGGAGCTGCACTC
Band lengths:
246-372
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]