BtaEX6011923 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000021310 | COL4A4
Description
collagen, type IV, alpha 4 [Source:HGNC Symbol;Acc:HGNC:2206]
Coordinates
chr2:116094610-116104632:-
Coord C1 exon
chr2:116104571-116104632
Coord A exon
chr2:116102783-116102825
Coord C2 exon
chr2:116094610-116094687
Length
43 bp
Sequences
Splice sites
3' ss Seq
GCATTTTTGTTTTCTTGTAGGTC
3' ss Score
11.15
5' ss Seq
GGGGTAAGT
5' ss Score
9.65
Exon sequences
Seq C1 exon
TTGCACATGGCACTGGTAAGGCGTCCTTTCAAGTGCAACAAGCTCTTGGCCACCAATTCCTG
Seq A exon
GTCACTCATACTTATTTTGTTTTCTGTACAATATGCATATGGG
Seq C2 exon
GGTGGAAAGAAGTTTATTGGTCTCTGTGGAGGAAGAGATTGCTCTCTTTGCCAGTGCTTTCCTGAAAAGGGATCTCGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000021310-'1-2,'1-0,2-2=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.077
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0139113=Collagen=PU(6.2=15.4)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCACATGGCACTGGTAAGGC
R:
TCCCTTTTCAGGAAAGCACTGG
Band lengths:
132-175
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]