MmuEX0012290 @ mm10
Exon Skipping
Gene
ENSMUSG00000067158 | Col4a4
Description
collagen, type IV, alpha 4 [Source:MGI Symbol;Acc:MGI:104687]
Coordinates
chr1:82563403-82571535:-
Coord C1 exon
chr1:82571369-82571535
Coord A exon
chr1:82569804-82569846
Coord C2 exon
chr1:82563403-82563480
Length
43 bp
Sequences
Splice sites
3' ss Seq
ATATTTTTGTTTTTCCATAGGTC
3' ss Score
12.58
5' ss Seq
GGGGTAAGT
5' ss Score
9.65
Exon sequences
Seq C1 exon
ATTCTAACAGGACACTGACTCCCGGCTGTCCATGAGAGTCTTCTGGAAGAGAGGAGAAGGCTGGAGGAGTATTTTGGGCACAGAGGTCACCCCTCGAGGGCACCAGTGCTCCAGGTCTTCATGAGGTGCTTTTTCAGATGGACCAAGTCCTTTGTCACAGCCCCCTG
Seq A exon
GTCACTAATATTTATCCTCTTTACTATACAATATGAATATGGG
Seq C2 exon
AGTGGCAAGAAGTATGGCGGCCCCTGCGGAGGAAGAAACTGCTCTGTCTGCCAGTGTTTTCCTGAGAAGGGATCTCGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000067158-'4-5,'4-4,5-5
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.064
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0139113=Collagen=PU(6.5=15.4)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATTTTGGGCACAGAGGTCACC
R:
GAGCAGTTTCTTCCTCCGCAG
Band lengths:
141-184
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types