BtaEX6021150 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000006828 | RAPGEF3
Description
Rap guanine nucleotide exchange factor (GEF) 3 [Source:HGNC Symbol;Acc:HGNC:16629]
Coordinates
chr5:32706961-32707771:+
Coord C1 exon
chr5:32706961-32707049
Coord A exon
chr5:32707352-32707430
Coord C2 exon
chr5:32707621-32707771
Length
79 bp
Sequences
Splice sites
3' ss Seq
CCCCTGTCCCTGCCTTGCAGAGA
3' ss Score
11.09
5' ss Seq
CCAATATCC
5' ss Score
-12.74
Exon sequences
Seq C1 exon
GTATACAGTGATGTCTGGCACCCCAGAGAAGATCCTAGAACTTCTGTTGGAGGCCATGCGGCCTGACTCCAGTGCTCATGACCCAACAG
Seq A exon
AGACATTCCTCAGTGACTTCCTCCTGACCCACAGTGTCTTCATGCCCACTGCCCAGCTCTGTGCTGCCCTCCTGCACCA
Seq C2 exon
CTTCCACGCGGAGCCCGCCGGGGGCAGCGAGCAGGAGCGCAGCTCCTACATATGCAACAAGAGGCAGCAGATCCTGCGGCTGGTCAGTCAGTGGGTGACCCTGTACGGCCCTACGCTCCGCACCGACCCTGTGGCCACCAGCTTCCTCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000006828-'15-17,'15-15,16-17=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.194 A=0.000 C2=0.137
Domain overlap (PFAM):
C1:
PF0061815=RasGEF_N=PU(25.5=90.3)
A:
PF0061815=RasGEF_N=FE(23.6=100)
C2:
PF0061815=RasGEF_N=FE(45.5=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGCACCCCAGAGAAGATCCTA
R:
TCACCCACTGACTGACCAGC
Band lengths:
171-250
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]