DreEX0007310 @ danRer10
Exon Skipping
Gene
ENSDARG00000075924 | RAPGEF4 (1 of 2)
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:HGNC Symbol;Acc:HGNC:16626]
Coordinates
chr6:6748946-6751611:+
Coord C1 exon
chr6:6748946-6749031
Coord A exon
chr6:6749262-6749340
Coord C2 exon
chr6:6751461-6751611
Length
79 bp
Sequences
Splice sites
3' ss Seq
TGCAAATGTTTCTCCCGCAGATC
3' ss Score
11.77
5' ss Seq
CCAATATCC
5' ss Score
-12.74
Exon sequences
Seq C1 exon
ATACACTGTGATGTCAGGCAGCCCTGAGAAGATTCTGGAGCATCTGCTGGAGACAATGCGACTGGACATTCATTTCTCTGACCCAG
Seq A exon
ATCCAGCTCTTGATGACTTTGTGCTCATGCACTGTGTTTTCATGCCCAACAGTCAGCTGTGCCCCGCTCTTCTGGCCCA
Seq C2 exon
CTATCACTCTCAGGCCTCTCAGGGCTCTGAGCAGGACCGTCTAGACTACGCTGTTGCCAGTAAGAGACGAGTGCTCAGCTTGGCTCTGCGCTGGGCTGCCCTGCAGGCCCACCATCTGCTGGAGGATGACACTGCGCTCGGCTTTCTGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000075924_MULTIEX1-4/17=3-5
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.039
Domain overlap (PFAM):
C1:
PF0061815=RasGEF_N=PU(24.8=90.0)
A:
PF0061815=RasGEF_N=FE(23.9=100)
C2:
PF0061815=RasGEF_N=FE(45.9=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGGAGACAATGCGACTGGAC
R:
GCAGTGTCATCCTCCAGCAGA
Band lengths:
175-254
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]