MmuEX6102148 @ mm10
Exon Skipping
Gene
ENSMUSG00000049044 | Rapgef4
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:MGI Symbol;Acc:MGI:1917723]
Coordinates
chr2:72205655-72223167:+
Coord C1 exon
chr2:72205655-72205743
Coord A exon
chr2:72208086-72208164
Coord C2 exon
chr2:72223017-72223167
Length
79 bp
Sequences
Splice sites
3' ss Seq
TCTCTTTTTTTCCCTTCCAGATT
3' ss Score
12.99
5' ss Seq
CCAATATCC
5' ss Score
-12.74
Exon sequences
Seq C1 exon
GTATACTGTGATGTCAGGAACACCTGAAAAGATTTTAGAGCATTTTCTAGAAACAATACGCCTTGAGCCATCGTTGAATGAAGCAACAG
Seq A exon
ATTCGGTTTTAAATGACTTTGTTATGATGCACTGTGTTTTTATGCCAAATACCCAGCTTTGCCCTGCCCTTGTGGCCCA
Seq C2 exon
TTACCACGCACAGCCTTCTCAAGGTACCGAGCAGGAGAGAATGGATTATGCCCTCAACAACAAGAGGCGGGTCATCCGCTTGGTCCTGCAGTGGGCGGCCATGTATGGCGATCTCCTCCAAGAAGATGATGTGGCCATGGCCTTCCTGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000049044-'63-39,'63-36,65-39=AN
Average complexity
A_S
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.161 A=0.000 C2=0.196
Domain overlap (PFAM):
C1:
PF0061815=RasGEF_N=PU(26.2=90.3)
A:
PF0061815=RasGEF_N=FE(24.3=100)
C2:
PF0061815=RasGEF_N=FE(46.7=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATACGCCTTGAGCCATCGTTG
R:
GCCATGGCCACATCATCTTCT
Band lengths:
175-254
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types