BtaEX6009007 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000020984 | RAPGEF4
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:HGNC Symbol;Acc:HGNC:16626]
Coordinates
chr2:23683382-23703295:-
Coord C1 exon
chr2:23703207-23703295
Coord A exon
chr2:23699666-23699744
Coord C2 exon
chr2:23683382-23683532
Length
79 bp
Sequences
Splice sites
3' ss Seq
TTTGTTTTCCTCCCCTGCAGATT
3' ss Score
12.8
5' ss Seq
CCAATATCC
5' ss Score
-12.74
Exon sequences
Seq C1 exon
GTATACTGTGATGTCAGGAACACCTGAAAAAATTTTAGAGCATTTTCTAGAAACGATACGCCTTGAGCCAGCTTTAAATGAAGCAACAG
Seq A exon
ATTCTGTTTTAAATGACTTTGTTATGATGCACTGTGTTTTTATGCCAAATACCCAGCTTTGCCCAGCTCTGGTGGCCCA
Seq C2 exon
TTACCATGCACAGCCTTCGCAAGGTACAGAACAAGAGAAAATGGATTATGCCCTCAACAACAAGAGGCGAGTCATCCGCCTGGTTTTGCAGTGGGCTGCCATGTATGGAGACATCCTACAAGAGGATGACGTGGCCATGGCTTTCCTGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000020984-'32-28,'32-27,33-28=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.161 A=0.000 C2=0.196
Domain overlap (PFAM):
C1:
PF0061815=RasGEF_N=PU(26.2=90.3)
A:
PF0061815=RasGEF_N=FE(24.3=100)
C2:
PF0061815=RasGEF_N=FE(46.7=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAAACGATACGCCTTGAGCCA
R:
TGGCCACGTCATCCTCTTGTA
Band lengths:
177-256
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]