RnoEX6017413 @ rn6
Exon Skipping
Gene
ENSRNOG00000001516 | Rapgef4
Description
Rap guanine nucleotide exchange factor 4 [Source:RGD Symbol;Acc:621886]
Coordinates
chr3:58874107-58890763:+
Coord C1 exon
chr3:58874107-58874195
Coord A exon
chr3:58876496-58876574
Coord C2 exon
chr3:58890613-58890763
Length
79 bp
Sequences
Splice sites
3' ss Seq
TTCTCTTTTCACCCTTCCAGATT
3' ss Score
11.28
5' ss Seq
CCAATATCC
5' ss Score
-12.74
Exon sequences
Seq C1 exon
GTATACTGTGATGTCAGGAACACCTGAAAAGATTTTAGAGCATTTTCTAGAAACAATACGCCTTGAGCCATCTTTGAATGAAGCAACAG
Seq A exon
ATTCAGTTTTAAATGACTTCGTTATGATGCACTGTGTTTTTATGCCAAACACCCAGCTTTGCCCCGCCCTCGTGGCCCA
Seq C2 exon
TTACCACGCACAGCCTTCTCAAGGTACAGAACAGGAGAGAATGGATTACGCCCTCAACAACAAGAGGCGGGTCATCCGCCTGGTTCTGCAATGGGCAGCCATGTATGGTGATCTTCTCCAAGAAGACGACGTGGCCATGGCTTTCCTGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000001516-'45-31,'45-29,47-31=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.161 A=0.000 C2=0.176
Domain overlap (PFAM):
C1:
PF0061815=RasGEF_N=PU(26.2=90.3)
A:
PF0061815=RasGEF_N=FE(24.3=100)
C2:
PF0061815=RasGEF_N=FE(46.7=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACGCCTTGAGCCATCTTTGAA
R:
CATGGCCACGTCGTCTTCTTG
Band lengths:
171-250
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]