MmuEX0038783 @ mm9
Exon Skipping
Gene
ENSMUSG00000022469 | Rapgef3
Description
Rap guanine nucleotide exchange factor (GEF) 3 [Source:MGI Symbol;Acc:MGI:2441741]
Coordinates
chr15:97580700-97588536:-
Coord C1 exon
chr15:97588448-97588536
Coord A exon
chr15:97588114-97588192
Coord C2 exon
chr15:97580700-97580825
Length
79 bp
Sequences
Splice sites
3' ss Seq
ACCCTAACCCTGCCCAACAGAGA
3' ss Score
5.62
5' ss Seq
CCAATATCC
5' ss Score
-12.74
Exon sequences
Seq C1 exon
GTATACGGTCATGTCTGGCACCCCAGAGAAAATCCTAGAACTGCTGTTGGAGGCTATGAGACCGGATTCCAGTGCTCATGACCCAACAG
Seq A exon
AGACGTTCCTCAGTGACTTCCTGCTGACCCACAGTGTCTTCATGCCCAGCACCCAGCTCTTCACTGCCCTCCTGCACCA
Seq C2 exon
ACCCCACACCCTGAGCAGCTGGGCCCCACTCTGGGTTCTTCTGAGATGCTGGACCTAGTGAGCGCCAAGGACCTGGCAGGCCAGCTGACCGACCATGACTGGAACCTCTTCAACAGGATCCACCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000022469_MULTIEX1-12/18=11-C2
Average complexity
C2*
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.290 A=0.007 C2=0.194
Domain overlap (PFAM):
C1:
PF0061815=RasGEF_N=PU(24.8=90.3)
A:
PF0061815=RasGEF_N=FE(23.0=100)
C2:
PF0061714=RasGEF=PU(12.9=57.1)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTTGGAGGCTATGAGACCGGA
R:
TGGTGGATCCTGTTGAAGAGGT
Band lengths:
169-248
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: