BtaEX6021157 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000006828 | RAPGEF3
Description
Rap guanine nucleotide exchange factor (GEF) 3 [Source:HGNC Symbol;Acc:HGNC:16629]
Coordinates
chr5:32703778-32704520:+
Coord C1 exon
chr5:32703778-32703884
Coord A exon
chr5:32704084-32704205
Coord C2 exon
chr5:32704352-32704520
Length
122 bp
Sequences
Splice sites
3' ss Seq
CTGTCCCCTTGTCTTCCCAGGCA
3' ss Score
10.7
5' ss Seq
ATGGTGAGC
5' ss Score
8.27
Exon sequences
Seq C1 exon
GCCTCCGCGGAGCGGGTGCTCAGGGCGGGAAAGCAGCTGCATCGACACCTCCTGGCCAACTGCCCCAACCTCATCCGAGACAGAAAGTACCACCTCCGGCTCCACCG
Seq A exon
GCAGTGCTGCTCCGGCCGGGAGCTGGTAGATGGGATCCTGGCCCTGGGGCTGGGCGTCCACTCCCGGAGCCAAGCCGTGGGTATCTGCCAGGTGCTCCTGGATGAAGGCGCCCTCTGCCATG
Seq C2 exon
TGAAACACGACTGGGCCTTCCAGGACCGAGACACCCAGTTCTACCGTTTCCCGGGGCCGGAGCCAGAGCCTGTGGGTGTCCATGAGCTAGAGGAAGAGCTGGTGGAGGCTCTGGCCCTGTTGTCCCAGCGGGGGCCCGATGCCCTGCTCACGGTGGCACTTCGAAAGCC
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000006828-'5-5,'5-3,6-5=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.105
Domain overlap (PFAM):
C1:
PF0061016=DEP=PU(18.3=36.1)
A:
PF0061016=DEP=FE(57.7=100)
C2:
PF0061016=DEP=PD(22.5=28.1)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGAAAGCAGCTGCATCGACA
R:
CTTTCGAAGTGCCACCGTGAG
Band lengths:
247-369
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]