DreEX6081739 @ danRer10
Exon Skipping
Gene
ENSDARG00000079291 | rapgef3
Description
Rap guanine nucleotide exchange factor (GEF) 3 [Source:ZFIN;Acc:ZDB-GENE-090312-38]
Coordinates
chr23:33487273-33488638:+
Coord C1 exon
chr23:33487273-33487379
Coord A exon
chr23:33488165-33488286
Coord C2 exon
chr23:33488458-33488638
Length
122 bp
Sequences
Splice sites
3' ss Seq
TTCTCTGGTTTGTCCCACAGACA
3' ss Score
10.96
5' ss Seq
ATGGTAAAT
5' ss Score
6.41
Exon sequences
Seq C1 exon
TTTTTATCAGATCGAATCCTGAAGGCTGCACGGGTAGTTTACAGCACTCTGATGGAGGGTAACCCAGGCCTGATAAGAGACAGAAAACACCATCTTAAGACCTACAG
Seq A exon
ACAGTGCTGCAGCGGAAAGGAGCTTGTGGATTGGTTAATGAAACTCAATGATTGTTTCCAGTCCCGGAGTCAGGCAGTCGGAATGTGGCAGGTTTTGGTGGATGAGGGCATTTTGGGTCATG
Seq C2 exon
TAAAACAGGAGTTAAACTTCCATGACAAAGACACACAGTTTTACCGCTTTATGGAAGCAGAGTTTGACCTCAATCACACAACTAACGAAAAAGACTCCAAAGACGACGAACTACAAGAGAGTTTATCGCTACTAATCCAGATGGGCCCAGATGCTCTTTTGACGATGATACTGCGTAAATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079291-'5-5,'5-4,6-5=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.115
Domain overlap (PFAM):
C1:
PF0061016=DEP=PU(17.1=33.3)
A:
PF0061016=DEP=FE(58.6=100)
C2:
PF0061016=DEP=PD(22.9=26.2)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCACGGGTAGTTTACAGCAC
R:
CAAAAGAGCATCTGGGCCCAT
Band lengths:
242-364
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]