BtaEX6063090 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000032544 | SPAG1
Description
sperm associated antigen 1 [Source:HGNC Symbol;Acc:HGNC:11212]
Coordinates
chr14:66330873-66339463:-
Coord C1 exon
chr14:66339311-66339463
Coord A exon
chr14:66334533-66334699
Coord C2 exon
chr14:66330873-66331005
Length
167 bp
Sequences
Splice sites
3' ss Seq
GAAACTGATTTTCTTTTTAGGAT
3' ss Score
9.28
5' ss Seq
CAGGTAGGG
5' ss Score
9.46
Exon sequences
Seq C1 exon
GGCCCTGGAGCTTCATCCATTCTCTATCAAGCCTCTTCTTCGACGAGCAATGGCCTATGAAACTGTAGAGCAGTATCAGAAAGCTTACGTGGATTATAAAACAGTGTTGCAAATAGACTGTGGAATCCAGTTAGCAAATGACAGTATTAACAG
Seq A exon
GATAACAAAGATTTTGATCGCTCTGGATGGCCCAAACTGGCGGGAGAAGCTGCCACCCATACCTGCTGTGCCCACCTCCACACAAGTCCGGGCTTGGCGGCCTGCAGCAGAGACGCCCCCAGACCAAGTGGGAGATTCCTGTTGCCATCCCCAGCCGGGCATGCCAG
Seq C2 exon
ATGAAAACGTATTTAAAACCCTTAAAGAAGAAGGAAATCAATGTGTAAAAGACAAAAACTATAAAGATGCCCTTAGTAAATACAGTGAATGCTTGAAGATTAACAACAAGGAATGTGCCATTTATACAAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000032544-'19-26,'19-24,21-26=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.386 C2=0.067
Domain overlap (PFAM):
C1:
PF134141=TPR_11=PD(14.6=11.5),PF0051523=TPR_1=WD(100=59.6)
A:
NO
C2:
PF134141=TPR_11=PU(60.9=93.3)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCCTCTTCTTCGACGAGCAAT
R:
TGGCACATTCCTTGTTGTTAATCT
Band lengths:
243-410
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]