GgaEX1054860 @ galGal4
Exon Skipping
Gene
ENSGALG00000016037 | SPAG1
Description
sperm associated antigen 1 [Source:HGNC Symbol;Acc:HGNC:11212]
Coordinates
chr2:128197023-128202867:+
Coord C1 exon
chr2:128197023-128197175
Coord A exon
chr2:128200043-128200218
Coord C2 exon
chr2:128202726-128202867
Length
176 bp
Sequences
Splice sites
3' ss Seq
ATGGGGCTTTTCCTTTTCAGAAT
3' ss Score
10.34
5' ss Seq
ATCGTATGT
5' ss Score
5.94
Exon sequences
Seq C1 exon
AGCTCTGGAGCTTCAGCCATTTTCCCTTAAGCCTCTTCTACGACGAGCAATGGCATATGAGTCTATGGAGCGGTATCGACAGGCATATGTTGATTACAAAACAGTCCTACAAATAGACAGCAGCATACAAGTAGCAAATGACAGTGTTAATAG
Seq A exon
AATAACGAAGACTTTAATTGATCAAGATGGTTCTAGTTGGAGGGAAAAACTGCCACCGATTCCAGTTGTCCCTATTGCTGCTCAGCTAAACAGGTGGGATGGAGGAAACTTTACTTCAGAGGTTAAGCCAAAAAGTCCCACAGATATCAACAAAGAAGAACAGTTGCAGATGAATC
Seq C2 exon
GTGAGAAAGCTGAGGAAAAGTTCAGGACATTAAAAAATGAAGGGAATGATTTTGTGAAGAAGGGTAAATATGATGAAGCTGTAAATAAATACAGTGAATGCTTGAAGTTAAATACCAAGGACTGCACAATATACACTAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000016037_MULTIEX1-3/5=1-4
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.350 C2=0.125
Domain overlap (PFAM):
C1:
PF134141=TPR_11=PD(7.9=11.5),PF0051523=TPR_1=WD(100=61.5)
A:
NO
C2:
PF134141=TPR_11=PU(60.0=87.5)
Main Skipping Isoform:
ENSGALT00000025838fB12317
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTTCTACGACGAGCAATGGCA
R:
AGTGTATATTGTGCAGTCCTTGGT
Band lengths:
256-432
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]