RnoEX6032022 @ rn6
Exon Skipping
Gene
ENSRNOG00000010078 | Spag1
Description
sperm associated antigen 1 [Source:RGD Symbol;Acc:1310702]
Coordinates
chr7:75040341-75046302:+
Coord C1 exon
chr7:75040341-75040493
Coord A exon
chr7:75043642-75043802
Coord C2 exon
chr7:75046170-75046302
Length
161 bp
Sequences
Splice sites
3' ss Seq
CAGTTCATTTTTCTCTTTAGAAT
3' ss Score
8.23
5' ss Seq
CAGGTAGGA
5' ss Score
9.79
Exon sequences
Seq C1 exon
GGCTCTGGAACTTCAGCCGTTCGCAGTGAAGCCTCTTTTACGGCGAGCGATGGCCTATGAGACTTTAGAGCAGTACAGGAGCGCGTATGTGGACTATATAACAGTGCTGAAGATAGACTGTAGGATCCAGCTGGCCAGCGACAGCGTTAACAG
Seq A exon
AATAACAAGAATCTTAACAGAGCTAGATGGACCAAAATGGCGGGAGAGACTGCCCCCCATCCCGGCTGTGCCTGCATCTGAGCCTCTGAGAGTTTGGCACCCTGCTGCTGAGACCCCAGACCAAGATCCGTGTCCCAACAGCTGCACGCCCACCATCACAG
Seq C2 exon
ATGAAAAAATGTTTCAAGCCCTTAAGGAAGAAGGAAATCAACTTGTAAAGGACAAAAATTATAAAGACGCCATTAGTAAATACAACGAATGCTTAAAGATTAACAGCAAGGCATGTGCCATCTATACAAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000010078-'23-18,'23-17,25-18=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.244 C2=0.007
Domain overlap (PFAM):
C1:
PF134141=TPR_11=PD(7.9=11.5)
A:
NO
C2:
PF134141=TPR_11=PU(61.8=93.3)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGTGAAGCCTCTTTTACGGCG
R:
GCACATGCCTTGCTGTTAATCT
Band lengths:
247-408
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]