MmuEX6053254 @ mm9
Exon Skipping
Gene
ENSMUSG00000037617 | Spag1
Description
sperm associated antigen 1 [Source:MGI Symbol;Acc:MGI:1349387]
Coordinates
chr15:36145922-36153996:+
Coord C1 exon
chr15:36145922-36146074
Coord A exon
chr15:36151161-36151321
Coord C2 exon
chr15:36153864-36153996
Length
161 bp
Sequences
Splice sites
3' ss Seq
ACAGTTCATTTTCTCTTTAGAAT
3' ss Score
8.32
5' ss Seq
CAGGTATGG
5' ss Score
9.99
Exon sequences
Seq C1 exon
GGCTCTGGAACTTCATCCGTTCTCAGTGAAGCCTCTTCTGCGGCGAGCAATGGCCTATGAGACCTTAGAGCAATACAGGAACGCATATGTGGACTATAAAACAGTGCTGCAGATAGATTGTGGGATCCAGCTGGCGAGCGACAGTGCTAACAG
Seq A exon
AATAGCACGAATCTTAACAGAGCTAGATGGATCAAAATGGCGGGAGAGACTGCCACCCATCCCAGCTGTACCCACATCTGAGCCACTGCGAGTTTGGCTCCCTGCTGCAGAGACCCCAGACCAAGATCCCTGTCCCAACAACTGCATGCCGAGCATCACAG
Seq C2 exon
ATGAAAAAATGTTTCAAGCCCTCAAGGAAGAAGGAAATCAACTTGTAAAGGACAAAAATTATAAAGATGCCATTAGTAAATACAATGAATGCTTAAAGATTAACAGCAAGGCATGTGCCATCTATACAAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000037617-'14-16,'14-14,15-16=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.152 C2=0.022
Domain overlap (PFAM):
C1:
PF134141=TPR_11=PD(7.9=11.5),PF0051523=TPR_1=WD(100=59.6)
A:
NO
C2:
PF134141=TPR_11=PU(61.8=93.3)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTCAGTGAAGCCTCTTCTGCG
R:
GCACATGCCTTGCTGTTAATCT
Band lengths:
250-411
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
Other AS DBs: