DreEX6072482 @ danRer10
Exon Skipping
Gene
ENSDARG00000004017 | spag1a
Description
sperm associated antigen 1a [Source:ZFIN;Acc:ZDB-GENE-030131-9443]
Coordinates
chr19:12073525-12084128:+
Coord C1 exon
chr19:12073525-12073677
Coord A exon
chr19:12076703-12076857
Coord C2 exon
chr19:12083987-12084128
Length
155 bp
Sequences
Splice sites
3' ss Seq
CGTCTGATATCTGCTTTCAGAAT
3' ss Score
8.65
5' ss Seq
AAGGTGACT
5' ss Score
6.64
Exon sequences
Seq C1 exon
GGCGCTGGAGCTCCACCCCTTCTCGCTGAAGCCCCTCCTCCGGCGAGCGATGGCCTATGAGTCTCTGGAGCGCTACAGGAAAGCCTATGTGGATTACAAAACAGTGCTGCAGATTGACATCAGTGTGCAGGCCGCTCACGACAGTGTACACAG
Seq A exon
AATCACTAAAATGCTGATTGAGCAGGACGGTCCTGACTGGCGAGAGAAGCTTCCAGAGATTCCAGCGGTTCCTCTTTCTGCTCAGCAGCACCGCAAAGAAGAGCCCAGCGCAGAGCTCCTGCAGGCCCGAGCAGAAAGAGCAGAACAGGAGAAAG
Seq C2 exon
CCAGAAAAGCAGAGGCTCGATTCACAATACTGAAACAAGAAGGAAATGAGCTGGTGAAGAACAGCCAGTTTCAAGGTGCCTCGGAAAAATACAGCGAGTGTCTGGCCATCAAACCCAACGAATGTGCCATTTACACCAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000004017-'2-3,'2-2,3-3=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.419 C2=0.052
Domain overlap (PFAM):
C1:
PF134141=TPR_11=PD(8.0=11.5),PF0051523=TPR_1=WD(100=59.6)
A:
NO
C2:
PF134141=TPR_11=PU(62.9=91.7)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTCCACCCCTTCTCGCTGAA
R:
GGCCAGACACTCGCTGTATTT
Band lengths:
250-405
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]