BtaEX6076679 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000003300 | MFGE8
Description
milk fat globule-EGF factor 8 protein [Source:HGNC Symbol;Acc:HGNC:7036]
Coordinates
chr21:20891992-20898349:-
Coord C1 exon
chr21:20898194-20898349
Coord A exon
chr21:20897139-20897283
Coord C2 exon
chr21:20891992-20892176
Length
145 bp
Sequences
Splice sites
3' ss Seq
ATCTGCCTCTTCCCTCCTAGATA
3' ss Score
10.15
5' ss Seq
ATGGTGAGT
5' ss Score
10.13
Exon sequences
Seq C1 exon
GTGAACCTGATGCGGAAGATGTGGGTGACGGGTGTGGTGACCCAGGGGGCCAGCCGCGCAGGGAGTGCTGAGTACCTGAAGACTTTTAAAGTGGCCTACAGCACCGATGGGCGCCAATTCCAGTTCATCCAGGTTGCAGGGCGGTCAGGAGATAAG
Seq A exon
ATATTTATAGGTAATGTGAACAACAGCGGCCTGAAGATTAACCTGTTTGATACCCCCTTGGAGACGCAGTATGTGAGACTGGTACCCATCATCTGCCACCGGGGCTGCACCCTCCGCTTTGAACTCCTTGGCTGTGAGTTGAATG
Seq C2 exon
GATGCACTGAACCCCTAGGCCTGAAGGATAATACCATCCCCAACAAGCAGATCACAGCCTCCAGCTACTACAAAACCTGGGGCCTGAGTGCCTTTAGCTGGTTTCCCTACTACGCACGACTGGATAATCAGGGCAAGTTCAACGCCTGGACCGCCCAGACCAACAGTGCCTCTGAGTGGCTGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000003300-'9-10,'9-8,11-10=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0075420=F5_F8_type_C=FE(36.7=100)
A:
PF0075420=F5_F8_type_C=PD(29.5=83.7)
C2:
PF0075420=F5_F8_type_C=PU(32.1=72.6)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTAAAGTGGCCTACAGCACCG
R:
ACTCAGAGGCACTGTTGGTCT
Band lengths:
248-393
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]