MmuEX6011631 @ mm10
Exon Skipping
Gene
ENSMUSG00000030605 | Mfge8
Description
milk fat globule-EGF factor 8 protein [Source:MGI Symbol;Acc:MGI:102768]
Coordinates
chr7:79136657-79142554:-
Coord C1 exon
chr7:79142402-79142554
Coord A exon
chr7:79141645-79141789
Coord C2 exon
chr7:79136657-79136841
Length
145 bp
Sequences
Splice sites
3' ss Seq
CCCTCCCCCTTCTCTTCTAGGAG
3' ss Score
12.95
5' ss Seq
ACGGTGAGT
5' ss Score
11.45
Exon sequences
Seq C1 exon
GTGAACCTTCTGCGGAAGATGCGGGTATCAGGTGTGATGACGCAGGGTGCCAGCCGTGCCGGGAGGGCGGAGTACCTGAAGACCTTCAAGGTGGCTTACAGCCTCGACGGACGCAAGTTTGAGTTCATCCAGGATGAAAGCGGTGGAGACAAG
Seq A exon
GAGTTTTTGGGTAACCTGGACAACAACAGCCTGAAGGTTAACATGTTCAACCCGACTCTGGAGGCACAGTACATAAAGCTGTACCCTGTTTCGTGCCACCGCGGCTGCACCCTCCGCTTCGAGCTCCTGGGCTGTGAGTTGCACG
Seq C2 exon
GATGTTCTGAGCCCCTGGGCCTGAAGAATAACACAATTCCTGACAGCCAGATGTCAGCCTCCAGCAGCTACAAGACATGGAACCTGCGTGCTTTTGGCTGGTACCCCCACTTGGGAAGGCTGGATAATCAGGGCAAGATCAATGCCTGGACGGCTCAGAGCAACAGTGCCAAGGAATGGCTGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000030605_MULTIEX1-5/5=4-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.015
Domain overlap (PFAM):
C1:
PF0075420=F5_F8_type_C=FE(36.2=100)
A:
PF0075420=F5_F8_type_C=PD(29.7=83.7)
C2:
PF0075420=F5_F8_type_C=PU(32.6=72.6)
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTCTGCGGAAGATGCGGGTAT
R:
CAAAAGCACGCAGGTTCCATG
Band lengths:
242-387
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types