DreEX6102136 @ danRer10
Exon Skipping
Gene
ENSDARG00000045803 | mfge8b
Description
milk fat globule-EGF factor 8 protein b [Source:ZFIN;Acc:ZDB-GENE-070209-137]
Coordinates
chr25:19081062-19083544:+
Coord C1 exon
chr25:19081062-19081217
Coord A exon
chr25:19082146-19082290
Coord C2 exon
chr25:19083360-19083544
Length
145 bp
Sequences
Splice sites
3' ss Seq
ATATTCATTGTGTCCTCCAGATT
3' ss Score
8.96
5' ss Seq
ACGGTAAAA
5' ss Score
6.53
Exon sequences
Seq C1 exon
GTCAACCTGCAGAGGAAGATGCGCTTCACTGGCATCATTACCCAGGGTGCCAGTCGCATGGGCACAGCCGAGTTCATCAAAGCCTTCAAGGTGGCGTCCAGTTTGGATGGAAGAACCTACACCATGTACAGACCTGAGGGCCAGAGCAAAGATATG
Seq A exon
ATTTTTGTTGGTAACATGGACAATGATGGGACAAAGACGAATTTGTTCGAGCCTCCAATCATCGCTCAGTTCCTCCGGATTGTTCCTGTGGTGTGCCGTAAAGCCTGTACTCTGAGAATGGAGCTGGTCGGCTGTGAACTCAACG
Seq C2 exon
GTTGCTCCGAGCCGCTCGGCGTCAAGTCTCGGCTCATCAGTGACCGGCAGATGACATCGTCCAGCGCTTTCCGCACCTGGGGCATTGAGGCCTTCACCTGGCACCCGCATTACGCCCGTCTGGACAAACAGGGCAAGACCAACGCCTGGACGGCAGCTACAAACAACCGCTCCGAGTGGCTGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000045803-'6-8,'6-6,7-8=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.061 C2=0.016
Domain overlap (PFAM):
C1:
PF0075420=F5_F8_type_C=FE(36.7=100)
A:
PF0075420=F5_F8_type_C=PD(29.5=83.7)
C2:
PF0075420=F5_F8_type_C=PU(32.1=72.6)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAGCCGAGTTCATCAAAGCCT
R:
TTGTTTGTAGCTGCCGTCCAG
Band lengths:
258-403
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]