RnoEX0054078 @ rn6
Exon Skipping
Gene
ENSRNOG00000017510 | Mfge8
Description
milk fat globule-EGF factor 8 protein [Source:RGD Symbol;Acc:3083]
Coordinates
chr1:140848458-140854620:-
Coord C1 exon
chr1:140854465-140854620
Coord A exon
chr1:140853745-140853889
Coord C2 exon
chr1:140848458-140848642
Length
145 bp
Sequences
Splice sites
3' ss Seq
CCCTGCCCCTTCTCTTGTAGGAG
3' ss Score
11.27
5' ss Seq
ATGGTGAGT
5' ss Score
10.13
Exon sequences
Seq C1 exon
GTGGACTTTCTGCGGAAGATGCGGGTATCAGGTGTGATGACACAGGGTGCCAGCCGTGCCGGGAGGGCGGAATACCTGAAGACCTTCAAGGTGGCTTACAGCCTCGATGGACGCAGGTTCGAGTTCATCCAGGATGAAAGCGGAACCGGAGACAAG
Seq A exon
GAGTTTATGGGTAACCAGGACAACAACAGCCTGAAGATTAACATGTTCAACCCCACTCTGGAGGCACAGTACATAAGGCTGTACCCTGTCTCGTGCCACCGCGGCTGCACCCTCCGCTTCGAGCTCCTGGGCTGCGAGTTGCATG
Seq C2 exon
GATGCTCTGAGCCCCTGGGCCTGAAGAATAACACGATTCCTGACAGCCAAATAACAGCCTCCAGCAGCTACAAGACGTGGAACCTGCGTGCCTTTGGCTGGTACCCCCACTTGGGGCGGCTGGACAATCAGGGCAAGATCAATGCCTGGACAGCTCAGAGCAACAGTGCCAAGGAATGGCTGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000017510-'18-10,'18-7,19-10
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.038 A=0.082 C2=0.016
Domain overlap (PFAM):
C1:
PF0075420=F5_F8_type_C=FE(36.7=100)
A:
PF0075420=F5_F8_type_C=PD(29.5=83.7)
C2:
PF0075420=F5_F8_type_C=PU(32.6=72.6)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGACTTTCTGCGGAAGATGC
R:
CGCAGGTTCCACGTCTTGTAG
Band lengths:
243-388
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]